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Microbial preparation for producing non-antigen soya bean waste and preparation thereof

A microbial inoculum, antigen-free technology, applied in the field of microbial applications, can solve the problem of not specifically introducing the technical content, and achieve the effects of reducing toxic effects, increasing the addition ratio, and improving palatability.

Inactive Publication Date: 2009-08-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the relevant reports of the Netherlands and my country’s Taiwan, the technical content was not introduced in detail.

Method used

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  • Microbial preparation for producing non-antigen soya bean waste and preparation thereof
  • Microbial preparation for producing non-antigen soya bean waste and preparation thereof
  • Microbial preparation for producing non-antigen soya bean waste and preparation thereof

Examples

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preparation example Construction

[0035] The preparation method of WB117 bacterial agent, carries out according to the following steps:

[0036] (1) Preparation of medium at all levels, wherein:

[0037] a) Preparation of PDA slant solid medium: 200 g of potatoes, 2 g of glucose, 2 g of agar, 1 liter of water, sterilized for later use;

[0038] b) Preparation of shake flask LB liquid medium: 5 g of beef extract, 10 g of peptone, 5 g of NaCl, 1 liter of water, adjust the pH to 7.2-7.4, and sterilize for later use;

[0039] c) Preparation of solid medium: by weight bran 80%, bentonite 5%, rice bran 8.5%, glucose 5% and K 2 HPO 4 1.5% was weighed and mixed well, then water was added in a ratio of 1:1 by weight of material and water, and sterilized at 0.01 MPa for 30 minutes to form a solid medium for later use;

[0040] (2) Cultivation of the slant strain: inoculate the bacterial strain WB117 on the PDA slant solid medium in step (1), cultivate it in a 30° C. incubator for 24 hours, take it out and apply it d...

Embodiment 2

[0044] Embodiment 2: (formula and preparation 2 of Bacillus subtilis WB117 bacterial agent)

[0045] In the present embodiment, the (weight) formula of the solid medium is: bran 70%, bentonite 15%, rice bran 13%, glucose 1% and K 2 HPO 4 1%; liquid strains in shake flasks were prepared by culturing for 20 hours in a shaker at 34°C with a speed of 120 rpm; solid parent species were inoculated at 2% inoculum and cultivated at 33°C for 65 hours; batches The product is inoculated with 0.5% of the weight of the prepared bacterial agent, and fermented and cultivated for 65 hours in the aerobic koji pool; the rest of the preparation methods are the same as in Example 1.

Embodiment 3

[0046] Embodiment 3: (formula and preparation 3 of Bacillus subtilis WB117 bacterial agent)

[0047] In the present embodiment, the (weight) formula of the solid medium is: bran 75%, bentonite 10%, rice bran 10%, glucose 2% and K 2 HPO 4 3%; shake flask liquid strains were prepared by culturing for 16 hours at 37°C in a shaker with a rotation speed of 170 rpm; solid parent species were inoculated at 3% inoculum and cultivated for 60 hours at 37°C; batches The product is inoculated with 2% of the weight of the prepared bacterial agent, and fermented and cultivated for 68 hours in a solid fermenter; the rest of the preparation methods are the same as in Example 1.

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Abstract

The invention discloses a microbial agent for producing nonantigenic soybean meal and a preparation method thereof and belongs to the technical field of microorganism application. The microbial agent uses Bacillus subtilis strain WB117, Saccharomyces cerevisiae Meyenex Hansen strain WY238 and Lactobacillus sp. strain L1 as strains. The strains are prepared into single-strain microbial agents respectively through inoculation, fermentation and culture; and the single-strain microbial agents are blended according a weight ratio of 1-3:1-3:1-3 to form a composite microbial agent. The microbial agent ferments a soybean meal raw material so as to remove antigenic substance and anti-nutritional factors, reduce the toxic action of the soybean meal on livestock, promote digestion and absorption, and improve percentage of the soybean meal in feed and the nutritional value of the soybean meal. The microbial agent and the method can be popularized among and used in feed additive enterprises and livestock farms or peasant households.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, in particular to a microbial agent for producing antigen-free soybean meal by using microorganisms and a preparation and application method thereof. Background technique [0002] Feed is the material basis of the aquaculture industry. Protein raw materials have always been the most critical component of feed because of their important nutritional and physiological functions. The particularity is also related to the quality and safety of livestock products, and even the health of meat consumers. Research and practice have proved that some animal proteins can transmit animal diseases, even zoonotic diseases, such as mad cow disease, which is related to the use of homologous meat and bone meal; some plant proteins, because of the existence of anti-nutritional factors, cause animal dyspepsia and diarrhea, affecting the healthy growth of animals. [0003] With the rapid development...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/14C12R1/125C12R1/865C12R1/225A23K10/12A23K10/37
CPCY02P60/87
Inventor 汤江武姚晓红吴逸飞王新
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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