Organ preservative fluid and preparation method thereof

Abstract

The invention relates to a preservation solution for organs, tissues or cells of human bodies or animals. The organ preservation solution comprises sodium citrate, potassium citrate, magnesium sulfate, sodium dihydrogen phosphate, sodium hydroxide, adenosine, arginine, tryptophan, mannitol, tetramethylpyrazine hydrochloride and other components. The organ preservation solution can be used for cooling, lavaging and preserving the organs of the human bodies or the animals, can effectively preserve human in vitro kidneys for 48 hours and animal in vitro kidneys for 72 hours, can prevent ischemia-reperfusion injury, and has great value for clinical application.

Description

Technical field: [0001] The present invention relates to medicine, in particular to a preservation solution for human or animal organs, tissues or cells, especially a preservation solution for human or animal organs. Background technique: [0002] Organ transplantation is a major advance in human medical science in the 20th century. Since its clinical application in the 1960s, transplantation medicine has developed by leaps and bounds. At present, the total number of transplants in the field of kidney, liver, heart, pancreas and bone marrow transplantation in the world has exceeded 700,000 cases (times), and the longest surviving organ transplant recipient has been nearly half a century. Organ transplantation has become one of the main directions of medical development in the 21st century, and it is an effective cure for end-stage organ failure. However, any clinical organ transplantation must first have a high-quality donor, which is the prerequisite and fundamental guaran...

AI Technical Summary

Problems solved by technology

[0007] However, this preservation solution also has disadvantages: 1. The concentration of magnesium sulfate is high, and it is easy to precipitate at low temperature, and it precipitates in the preservation solution and renal blood vessels to cause tissue damage; 2. It does not contain effective buffer pairs, and the pH value is unstable. Time (more than 24 hours) is not enough to prevent cell acidification when storing the kidney; 3. It contains more biochemical agents and the purity is not enough

Although UW liquid has been widely used in the world, its formula liquid has high viscosity, so the cooling speed is slow; the potassium ion content is too high, improper use will cause cardiac arrest in the operation, and the price is very expensive, so it is not suitable universal application

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