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Isolated liver perfusion system and method

An isolated liver technology, applied in the field of liver transplantation, can solve the problems that the static cold preservation technology cannot meet the preservation requirements of marginal donor livers, and achieve the effects of facilitating quality evaluation, ensuring life activity, and alleviating reperfusion injury

Active Publication Date: 2016-06-15
杭州莱普晟医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides an isolated liver perfusion system, which solves the problem that the existing static cold preservation technology cannot meet the preservation requirements of marginal donor livers

Method used

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  • Isolated liver perfusion system and method
  • Isolated liver perfusion system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Embodiment 1 isolated liver perfusion system

[0093] Such as figure 1 As shown, an isolated liver perfusion system in this embodiment includes a perfusate temperature-controlled tank 1 for containing perfusate and an isolated liver storage tank 2 for placing an isolated liver, a perfusate temperature-controlled tank 1 and an isolated liver. Portal vein perfusion pathways, hepatic artery perfusion pathways and perfusate return pathways are provided between the liver preservation tanks 2 .

[0094] Wherein, the perfusate temperature control tank 1 includes a water bath 3 and an inner tank 4 installed in the water bath 3 for holding the perfusate.

[0095] Depend on figure 1 It can be seen that an air inlet is provided on the inner tank 4, and the oxygenation module 5 is connected with the inner tank 4 through the air inlet, and the oxygenation module 5 is used for oxygenating the perfusate so that the perfusate can provide sufficient of oxygen.

[0096] The oxygenati...

Embodiment 2

[0110] Example 2 Using normal saline as the perfusion fluid to perfuse the isolated liver

[0111] In this embodiment, domestic pigs are taken as an example to test the preservation effect of the isolated liver perfusion system in Example 1 on the isolated liver of domestic pigs.

[0112] Anesthetize domestic pigs, make a cross incision into the abdomen and disinfect, separate and free the abdominal aorta, inferior vena cava, and portal vein, and perfuse the portal vein and abdominal aorta with heparinized saline and Shenbao liquid after catheterization; cut the gallbladder, gallbladder The main tube was cut off, the diaphragm was opened, the thoracoabdominal aorta was cut off into the chest cavity, the gastrohepatic ligament was cut off, and the donor liver was removed; after repairing the liver, the isolated liver perfusion system in Example 1 was used for in vitro preservation.

[0113] The method for perfusing the isolated liver of domestic pigs using the isolated liver pe...

Embodiment 3

[0125] Example 3 Using UW as the perfusion fluid for isolated liver perfusion

[0126] The same method as in Example 2 was used to perfuse the isolated pig liver, but UW solution was used as the perfusion fluid.

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Abstract

The invention discloses an isolated liver perfusion system and method. The system comprises a perfusate temperature control tank, an isolated liver preservation tank, a portal vein perfusion path, a hepatic artery perfusion path, a perfusate backflow path and a bile collector. The portal vein perfusion path is used for conveying perfusate to the portal vein of an isolated liver, the hepatic artery perfusion path is used for conveying perfusate to the hepatic artery of the isolated liver, the perfusate backflow path is used for recycling perfusate flowing out of the isolated liver into the perfusate temperature control tank, and the bile collector is connected with the isolated liver preservation tank and used for collecting bile. The isolated liver is perfused from the portal vein and the hepatic artery through the two paths respectively, the system and method are applicable to damage protection in the isolated liver preservation process, the reperfusion injury of the liver can be significantly relieved, and reliability, stability and repeatability are high. The system and method can effectively store the isolated liver at low temperature, mild hypothermia and normal temperature, and the limitation that the isolated liver needs to be stored at low temperature and the requirement for marginal donor liver preservation cannot be met in the prior art is changed.

Description

technical field [0001] The invention belongs to the technical field of liver transplantation, and in particular relates to an isolated liver perfusion system and an isolated liver perfusion method. Background technique [0002] Liver transplantation is the most effective method for the treatment of end-stage liver disease. With the continuous development of surgical techniques, new immunosuppressants and imaging techniques, the survival rate of recipients after liver transplantation continues to increase. Although applications such as donation after cardiac death (DCD) and split livers have expanded the source of liver donors, compared with the ever-increasing waiting list for liver transplantation, the source of liver donors is still seriously insufficient (O'MahonyCA, GossJA.Thefutureoflivertransplantation.TexHeartInstJ , 2013,39(6):874-875.). [0003] At present, the traditional static cold storage (SCS) method (GuibertEE, PetrenkoAY, BalabanCL, et al. Organ perservation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0247
Inventor 李建辉谢海洋贾俊君胡贵权陈旭东郑骏姜骊周燕飞张静周琳郑树森
Owner 杭州莱普晟医疗科技有限公司
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