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Additive for Cordyceps militaris fluid nutrient medium

A technology of Cordyceps militaris and liquid culture medium, which is applied in the direction of adding compounds to stimulate growth, fungi, etc., can solve the problems of long fermentation period, slow production of culture medium strains, unable to meet the needs of production, etc., and achieves the increase of cordycepic acid content , The effect of promoting the synthesis of cordycepic acid and increasing the yield

Inactive Publication Date: 2009-07-08
吉林睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing medium strains have slow production and long fermentation cycle, which cannot meet the production needs

Method used

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  • Additive for Cordyceps militaris fluid nutrient medium
  • Additive for Cordyceps militaris fluid nutrient medium
  • Additive for Cordyceps militaris fluid nutrient medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Sucrose 20g / L, peptone 10g / L, yeast extract powder 10g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 3g / L, vitamin B10.100g / L, zinc chloride 0.01g / L, ammonium chloride 10g / L, packed in a 250ml Erlenmeyer flask with a liquid volume of 100ml, sterilized at 121°C for 20 minutes to make a culture medium.

Embodiment 2

[0026] Sucrose 20g / L, peptone 10g / L, yeast extract powder 10g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 3g / L, vitamin B10.100g / L, zinc chloride 0.01g / L, olive oil 10ml / L, gibberellin 0.001g / L, ammonium chloride 10g / L, packed in a 250ml Erlenmeyer flask with a liquid volume of 100ml, sterilized at 121°C for 20 minutes to make a culture medium.

Embodiment 3

[0028] Sucrose 20g / L, peptone 10g / L, yeast extract powder 10g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 3g / L, vitamin B10.100g / L, zinc chloride 0.012g / L, olive oil 12ml / L, gibberellin 0.0011g / L, ammonium chloride 14g / L, packed in a 250ml Erlenmeyer flask with a liquid volume of 100ml, sterilized at 121°C for 20 minutes to make a culture medium.

[0029] table 3

[0030]

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PUM

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Abstract

The invention is an additive for cordyceps liquid culture medium, which relates to the optimization of fluid culture north cordyceps culture medium components, increasing the mycelium dry weight of the north cordyceps abd gte secondary metabolite output by adding zinc chloride, olive oil, growth hormone and ammonium muriate. The invention has positive effects of: changing the culture medium components based on the ordinary north cordyceps fluid culture, promoting the physiological metabolism function of the north cordyceps, quickening the growth velocity and the synthesis of the metabolite cordycepic acid, wherein, the mycelium dry weight can reach about 16.8g / L, the content of can reach 1.375g / L and above, and the adenosine content can achieve more than 80mg / L.

Description

technical field [0001] The invention provides an additive for liquid culture medium of Cordyceps militaris, relates to formula optimization of liquid culture of Cordyceps militaris, effectively improves the output of liquid cultured mycelium and the content of some active ingredients, and belongs to the technical field of biological bacteria cultivation. Background technique [0002] With the improvement of people's living standards and the enhancement of health care awareness, the demand for Cordyceps sinensis, one of the main raw materials for health care and medicine, is increasing day by day. In addition, the collection of Cordyceps sinensis has seriously damaged wild vegetation. Cordyceps is a wild species resource that is prohibited from being collected in the wild and protected by the state. In order to meet the needs of the market, artificial cultivation of Cordyceps militaris has become the current development trend. At present, the cordyceps miltitaris strain is w...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/14
Inventor 孟繁宇
Owner 吉林睿康生物科技有限公司
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