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Magnetic particle micro array apparatus for high throughput detecting biomacromolecule and its use method

A technology of biological macromolecules and magnetic particles, which is applied in biological testing, analytical materials, material inspection products, etc., can solve problems such as complex operation of biochips, complex chemical modification of glass slides, complex fixation process, etc., to reduce experimental costs and detect The effect of a fast and simplified preparation process

Active Publication Date: 2009-06-17
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this kind of biochip preparation method, the glass slide as a solid phase carrier often needs complex chemical modification; at the same time, the immobilization of biological macromolecules on the surface of the functionalized carrier often requires a complicated immobilization process.
In addition, before the biochip is tested, it is often necessary to purify and concentrate the sample to be tested, which makes the operation of the biochip complicated and the cost is high

Method used

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  • Magnetic particle micro array apparatus for high throughput detecting biomacromolecule and its use method
  • Magnetic particle micro array apparatus for high throughput detecting biomacromolecule and its use method

Examples

Experimental program
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Effect test

Embodiment 1

[0016] A magnetic particle microarray device for detecting high-throughput biomacromolecules, including a microfluidic reaction cell cover sheet 1, a microarray composed of a sample to be detected-biomacromolecule-magnetic particle complex 2, polydimethylsiloxane Microfluidic reaction pool 3, slide glass 4 and magnet 5, described microfluidic reaction pool cover sheet 1 is provided with buffer solution inlet 6 and buffer solution outlet 7, and microfluidic reaction pool cover sheet 1 is arranged on polydimethylsiloxane Above the oxane microfluidic reaction pool 3, the buffer inlet 6 and the buffer outlet 7 communicate with the polydimethylsiloxane microfluidic reaction pool 3, and the microarray composed of the sample to be detected-biological macromolecule-magnetic particle complex 2 is set in the polydimethylsiloxane microfluidic reaction pool 3 , the glass slide 4 is set under the polydimethylsiloxane microfluidic reaction pool 3 , and the magnet 5 is set under the glass sli...

Embodiment 2

[0018] A method for using a magnetic particle microarray device for detecting high-throughput biomacromolecules. A sample to be detected is added to magnetic particles immobilized with biomacromolecules, and the molecules to be detected are combined with biomacromolecules connected to the surface of the magnetic particles. Get the sample-biological macromolecule-magnetic particle complex on the surface of the magnetic particle; put the sample-biological macromolecule-magnetic particle complex through the spotting device, and spot the sample into the polydimethylsiloxane microfluidic reaction cell , forming a microarray composed of the sample to be detected-biological macromolecule-magnetic particle complex, the microarray is magnetically fixed by the magnet; after the polydimethylsiloxane microfluidic reaction cell is sealed by the microfluidic reaction cell cover, Add fluorescently labeled probes or fluorescently labeled antibodies to the dimethylsiloxane microfluidic reaction...

Embodiment 3

[0020] Preparation of Magnetic Particle Microarray:

[0021] Schematic diagram of magnetic particle microarray attached figure 1 As shown, it includes slide glass, permanent magnet, polydimethylsiloxane (PDMS) microfluidic reaction cell (for the preparation method see: Pantoja et al, Biosensors and Bioelectronics, 2004, vol 20, 3, 509-517), The magnetic particle and the cover sheet of the microfluidic reaction pool are composed of five parts. Wherein, a magnet is used to adhere to the back side of the sample area of ​​the glass slide, and the magnetic particles are sampled in a microfluidic reaction cell prepared by PDMS. There are buffer inlets and outlets on the microfluidic coverslip, allowing various buffers and reaction solutions to flow into and out of the microfluidic reaction pool.

[0022] SNP Typing Using Magnetic Particle Microarrays

[0023] 1. In this example, we take the detection of the MTHFR gene (methylenetetrahydrofolate reductase gene) C677T SNP site as a...

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Abstract

The invention discloses a biomacromolecule detection platform of a magnetic particle microarray, which is established by combining magnetic nanoparticles and biochips with microfluidics technology. The magnetic nanoparticles are taken as a biomacromolecule carrier in the magnetic particle microarray. A DNA probe or an antibody is fixed on the surface of the magnetic particles to allow the magnetic particles to cross or react with a sample to be detected, thus various samples to be detected are fixed on the surface of the magnetic particles, and then the magnetic particles are spotted in a microfluid reaction cell with a magnetic substrate to form the magnetic particle microarray. A fluorescent probe or a fluorescent labeled antibody is added to the microfluid reaction cell and reacts in the reaction cell, and an obtained mixture is washed, thus obtaining information of various samples by a fluorescent biochip scanner.

Description

1. Technical field [0001] The invention belongs to the technical field of biological information detection, in particular to a magnetic particle microarray device for detecting high-throughput biological macromolecules and a method for using the same. 2. Background technology [0002] With the development of science and technology and people's continuous attention to health issues, people increasingly need to be able to conveniently and quickly perform various detections on biomacromolecules of interest, which prompts people to develop various biological detection methods methods to meet the needs of different fields. [0003] Since the 1990s, more and more biochip technology has attracted people's attention. Biochip chip technology achieves accurate, rapid and large-scale information analysis of compounds, proteins, nucleic acids, cells or other biological components by immobilizing a large number of molecular recognition probes on the surface of a tiny substrate, or const...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N35/00
Inventor 李松刘洪娜何农跃李智洋
Owner SOUTHEAST UNIV
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