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Rhamnose bacterium lacticum Pr2 peptide and coding gene thereof, separation method and application

A technology of Lactobacillus rhamnosus and genes, applied in the field of immunomedicine, can solve the problems of poorly understood cellular and molecular mechanisms, and unknown properties of probiotic immune-modulating components.

Inactive Publication Date: 2011-06-22
郑鹏远 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, despite some progress in this area, the nature of the immunomodulatory components of probiotics remains poorly understood, and the cellular and molecular mechanisms underlying the immunosuppressive effects of probiotics are poorly understood

Method used

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  • Rhamnose bacterium lacticum Pr2 peptide and coding gene thereof, separation method and application
  • Rhamnose bacterium lacticum Pr2 peptide and coding gene thereof, separation method and application
  • Rhamnose bacterium lacticum Pr2 peptide and coding gene thereof, separation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Purification of Lactobacillus rhamnosus Pr2 peptide

[0049] 1. Accumulated evidence shows that the application of probiotics has a regulatory effect on immune function, for example, repairing unbalanced Th1 / Th2 function, improving the production of Tregs, etc. Because live bacteria are necessary to obtain therapeutic effects, the inventors of the present invention speculated that a component in the culture supernatant of probiotics may play a role in regulating the function of DCs and further regulating the immune response. The inventors of the present invention used bone marrow-derived dendritic cells (BMDCs) as an evaluation system, and the culture supernatant precipitate of Lactobacillus rhamnosus was used to stimulate the growth of BMDCs. Since DCs-derived IL-10 and transforming growth factor (TGF-β) play a key role in the production of Tregs, the inventors of the present invention have studied the culture process of BMDCs stimulated by the culture sup...

Embodiment 2

[0058] Example 2 Functional detection of Lactobacillus rhamnosus Pr2 peptide

[0059] 1. The role of Pr2 in increasing the expression of IL-10 in BMDCs.

[0060] After stimulation by Pr2, the expression levels of IL-10 in the culture medium and BMDCs cell extracts were significantly increased compared with the expression levels after stimulation by the supernatant precipitate (Fig. 1A, B), and it was dose-dependent ( Figure 1E , F). The dynamic observation of IL-10 expression in BMDCs, continuous observation studies showed that Pr2 increased the expression of IL-10 reached a peak at 12 hours, and maintained a high level throughout the observation period (96 hours) ( Figure 1G ).

[0061] 2. Whether Stat3 is involved in the above Pr2-mediated BMDCs expression of IL-10

[0062] Studies have shown that Stat3 may play an important role in the expression of IL-10. The inventors of the present invention also investigated whether Stat3 is involved in the above Pr2-mediated expr...

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Abstract

The invention relates to the field of immune medicine, in particular to a rhamnose lactobacillus Pr2 peptide and a coding gene, a separation method and application thereof. The rhamnose lactobacillus Pr2 peptide has an amino acid sequence shown as SEQ No.1. The rhamnose lactobacillus sourced Pr2 peptide has the capability of producing Tregs through in-vitro stimulus. In addition, the Tregs can produce antigenic specificity Tregs when being exposed under Pr2 and antigen. The antigenic specificity Tregs can be activated when being exposed under specificity antigen. The activated antigenic specificity Tregs can release functional molecules such as IL-10, TGF- beta and FasL. The adoptive transfer of the antigenic specificity Tregs to a sensitized mouse or the giving of the Pr2 and specificityantigen to the sensitized mouse can obviously inhibit the antigenic specificity Th2 reaction and anaphylactic reaction in the body of the mouse. Therefore, the method has the potential application value in the treatment of food allergy diseases.

Description

technical field [0001] The present invention relates to the field of immunomedicine, in particular, the present invention relates to a Lactobacillus rhamnosus Pr2 peptide, its coding gene, its separation method and its application. Background technique [0002] Regulatory T cells (Tregs) play an important role in regulating T cell responses. Tregs can arise both inside and outside the thymus. Such cells express the transcription factors Foxp3, CD4, CD25, glucocorticoid-induced tumor necrosis factor receptor and cytotoxic T lymphocyte antigen-4. The suppressive function of active Tregs depends at least in part on their expression of TGF-β, IL-10, cytotoxic T lymphocyte antigen-4 or FasL. These molecules are capable of causing target cell death by inducing apoptosis. In atopy, dysfunction of allergen-specific Tregs is thought to be associated with allergen-specific Th2 responses and allergic inflammation. A large number of research reports also show that Tregs play an impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/195C12N15/31C07K1/18C07K1/20A61K39/02A61P37/08
Inventor 郑鹏远杨平常
Owner 郑鹏远
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