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Method for detecting cancer of stomach prognosis and staging blood serum protein

A protein and gastric cancer technology, applied in the field of malignant tumor detection, can solve the problems of insoluble protein electrophoresis separation and detection difficulties, and achieve the effects of saving medical costs, poor prognosis and improving efficiency

Inactive Publication Date: 2009-06-10
ZHEJIANG UNIV
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Problems solved by technology

In the past, proteomics research mainly relied on two-dimensional electrophoresis (Two Dimensional Electrophoresis, 2DE), and the number of detected proteins was less than the estimated total protein in the cell. However, the general 2DE can distinguish 1000-3000 protein spots, which is not suitable for low-copy proteins in cells. However, the electrophoretic separation and detection of extremely acidic or basic proteins, proteins with too large or too small molecular weight, and insoluble proteins (including membrane proteins) are still facing great difficulties

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  • Method for detecting cancer of stomach prognosis and staging blood serum protein
  • Method for detecting cancer of stomach prognosis and staging blood serum protein
  • Method for detecting cancer of stomach prognosis and staging blood serum protein

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Embodiment 1

[0020] Embodiment one: detection method of the present invention

[0021] 1. Serum sample preparation:

[0022] (1) All serum samples were thawed in an ice bath for 30-60 minutes and then centrifuged for 5 minutes. Take 10ul serum and 20ul denaturation buffer U9 (9M Urea, 2% CHARPS, 50mM Tris-HCl PH9) and mix thoroughly. Medium shaking for 30 minutes;

[0023] (2) Carefully take out the SAX chip and mark it, put it into the biochip processor, add 200ul SAX binding buffer to each well and shake twice to pretreat the chip;

[0024] (3) Take 10ul of the sample and 110ul of SAX binding buffer (50mM Tris-HCl pH 9) solution and mix quickly to avoid bubbles, add 100ul of the processed serum sample to each well, and shake at 4°C for 60 minutes;

[0025] (4) After serum samples, add 200ul SAX binding buffer to each well, shake twice at room temperature, then add 200ul deionized water to wash the chip twice;

[0026] (5) Take out the chip, add 0.5ul SPA to each well after micro-dryin...

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Abstract

The invention provides a method of detecting cancer of stomach, judging prognosis and stage in blood serum, comprising: (1) mensurating a proteid icon of blood serum sample between tumor patients and healthy persons through a surface enhanced laser desorption / ionization-time of flight mass spectrometry (SELDI); (2) screening out corresponding tumor marker and building a detecting model for analyzing detection combined with a method of bioinformatics. The inventive method provides a serum protein profiling for conveniently and efficiently detecting cancer of stomach, increases efficiency of screening cancer of stomach, saves medical cost. Meanwhile for the cancer of stomach patients with confirmed diagnosis, the invention provides a serology method of differentiating bad prognosis and late stage, for guiding patients for treatment. At the same time, the invention provides markers for further research.

Description

technical field [0001] The present invention belongs to the detection method of malignant tumor, relates to the method of prognosis judgment and staging. Tumor markers were used for further studies. Background technique [0002] Gastric cancer is the second most common malignancy worldwide and remains an important cause of cancer death. In my country, the incidence rate of gastric cancer is about 20 / 100,000, and the death rate ranks second in the cancer mortality rate in my country. Most of the patients are in the middle and late stage, and the prognosis is poor. According to statistics, the 5-year survival rates of radical surgery for early and advanced gastric cancer are 90% and 40%, respectively. Therefore, only early detection, early diagnosis, and early treatment can benefit more gastric cancer patients. At present, the detection rate of early gastric cancer in my country is about 10%, so the screening of large-scale population is not only inefficient, but also a wast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N33/48
Inventor 黄建邱福铭余捷凯金祁峰郑树
Owner ZHEJIANG UNIV
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