Northeast wood frog and molecular identification method for egg oil thereof
A molecular and analytical system technology, applied in the field of molecular identification of Northeast Rana species and its product egg oil, to achieve reliable detection results, strong specificity, and advanced and reasonable implementation steps
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[0050] The authenticity of the Northeast forest frog and egg oil purchased from Changbai Mountain in Jilin were used as identification objects.
[0051] Three samples of Rana and egg oil were taken respectively. The specific operation steps are as follows:
[0052] (1) Genomic DNA extraction:
[0053] ①Use an electronic balance (FA1604N, Shanghai Precision Scientific Instrument Factory) to weigh 200 mg of sample tissue (the muscle tissue of the Northeast Rana sample), wash it with 70% alcohol, cut it into pieces with sterilized scissors, put it into a 1.5ml EP tube, and naturally Air dry for 30-60 minutes to evaporate the alcohol;
[0054] ②Add 600 μl of 1×SET solution, 20 μl of proteinase K (10 mg / ml), and 25 μl of SDS to the EP tube, and use an air bath shaker (CHA-S, Shenzhen Tiannanhaibei Co., Ltd.) at 55°C for 2 hours;
[0055] ③ Add 500 μl Tris saturated phenol and 200 μl TE to the EP tube and invert up and down for 10 minutes;
[0056] ④Use a high-speed centrifuge (...
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