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Bursa pentapeptide, deriving peptide thereof and use thereof

A technology of bursa and derived peptides, applied in the field of molecular biology, can solve the problems of insufficient research on immune active factors of bursa, achieve the effect of improving the level of humoral immunity and cellular immunity, small molecular weight, and unsuitable for pollution

Active Publication Date: 2009-05-20
李德元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many studies on the immune activity factors of the bursa that have the same status as the thymus

Method used

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  • Bursa pentapeptide, deriving peptide thereof and use thereof
  • Bursa pentapeptide, deriving peptide thereof and use thereof
  • Bursa pentapeptide, deriving peptide thereof and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the preparation of bursin pentapeptide (Bp5)

[0035] Bursa pentapeptide (Bp5) can be biochemically extracted from chicken or other poultry bursa, and can also be obtained by artificial synthesis.

[0036] 1) Extracted from the bursa of Fabricius

[0037] Take 1000 g of fresh chicken bursa tissue from which fascia and adipose tissue have been removed, and wash once with 0.85% saline in mass concentration pre-cooled to 4-10°C. Drain and place in a homogenizer, then add 0.85% normal saline at 4-10°C and homogenize at high speed in a tissue masher three times at 0-10°C, 30 seconds each time. Sonicate the homogenate twice at 4°C, 5 minutes each time. Heat the lysate in a water bath to 80°C (5 minutes), quickly cool it in an ice bath to below 10°C, and centrifuge at 4,000rpm for 30min at 4°C. The resulting supernatants were combined and freeze-thawed twice at -20°C. Centrifuge at 12,000 rpm for 30 min at 4°C, and collect the supernatant. The supernatant wa...

Embodiment 2

[0039] Example 2 The immunomodulatory activity of bursin pentapeptide (Bp5)

[0040] Natural bursin pentapeptide has the ability to enhance the proliferation of mitogen-induced murine splenic T lymphocytes and B lymphocytes. On this basis, the inventors used 4-6 week-old mice as animal models, and used H9 subtype avian influenza virus as an antigen to immunize with different concentrations of bursin pentapeptide. Three times, the immunomodulatory function of bursin pentapeptide was analyzed (see attached table 1). Through the following experiments, it can be found that bursin pentapeptide can not only improve the body's humoral immune function but also improve the body's cellular immune function, and has the function of balancing Th1 and Th2 type immune responses.

[0041] Table 1

[0042] Groups immunized antigen and Bp5 dosage

(10mice / group) 1 BPS 0.1ml

2 AIV 0.1ml

3 AIV+high doses of Bp5 0.1ml+500ug

4 AIV+middle doses of Bp5 0.1ml+100ug

5 ...

Embodiment 3

[0063] Example 3 The antioxidant activity of bursin pentapeptide

[0064] Due to the unique structure of bursin pentapeptide, bursin pentapeptide has a very good antioxidant function, which is mainly manifested in the following aspects:

[0065] 1) Bursulin pentapeptide has a strong reducing ability (see attached Figure 6 )

[0066] The FRAP method can be used to detect that the pentapeptide has a very effective reducing ability. Thus it is revealed that vesicin pentapeptide has the characteristics of electron donor and thus has the ability to neutralize free radicals.

[0067] 2) Bursulin pentapeptide has the ability to resist liposome peroxidation (see attached Figure 7 )

[0068] TBARS method was used to evaluate the anti-liposome peroxidation ability of bursin pentapeptide. It can be seen from the figure that vesicin pentapeptide can very effectively inhibit the 2+ The peroxidation of the lecithin dispersion system was induced, and it was enhanced with the increase o...

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Abstract

The invention relates to a bursal pentapeptide, and the structural sequence of amino acid of the bursal pentapeptide is Cys-Lys-Asp-Val-Tyr. The bursal pentapeptide (Bp5) has simple structure, small molecular weight, no chemical toxicity and no immunogenicity, is easy to be prepared, and can not only be extracted from the bursa of fabricius of chicken or other poultry, but also be prepared by chemosynthesis with quite low cost in large-scale. The bursal pentapeptide (Bp5) can promote the multiplication of T lymphocyte and B lymphocyte, improve the humoral immunity level and cellular immunity level of an organism as well as the capability of anti-peroxidation stress of the organism, is a medicament or preparation with wide application prospect and the functions of immunoregulation, immunotherapy and anti-peroxidation, and can be applied to the fields such as fundamental research, clinical treatment, nutrition and health care, cosmetics, and the like.

Description

1. Technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a bursal pentapeptide with immunoregulatory activity and antioxidant activity, its derived peptide and application thereof. 2. Background technology [0002] Prior art: biologically active peptides (Biologically active peptides, BAP) refer to a class of polypeptides with a molecular weight of less than 6000 D and multiple biological functions. Its molecular structure varies in complexity, ranging from simple dipeptides to circular macromolecular polypeptides, and these polypeptides can be modified by phosphorylation, glycosylation or acylation. There are many kinds of active peptides, which widely exist in animals and have important physiological functions, ranging from immune regulation, anti-oxidation, hormone-like, antibiotic-like to seasoning and changing the taste of feed. For example, immunoactive peptides can not only enhance the immune ability of...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61K8/64A61P37/04A61P35/00A61P31/12A61P39/06A61P17/18A61Q1/00A23L1/29A23K1/16A23K20/147A23L33/18
Inventor 李德元陈溥言耿志荣
Owner 李德元
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