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Method for extracting sulfabenzpyrazine from animal sample and special immune affinity sorbent thereof

A single technology of sulfaquinoxaline and sulfaquinoxaline, which is applied in the field of extracting sulfaquinoxaline, can solve the problems of no commercial IAC column for sale, waste of organic solvents, cumbersome treatment process, etc., and reduce analysis cost and environment Pollution, simplified pre-treatment process, good purification effect

Inactive Publication Date: 2010-11-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pretreatment of these methods utilizes liquid-liquid distribution, conventional SPE column purification and separation, all of which have shortcomings such as cumbersome treatment process, poor purification effect, waste of organic solvents, and long time required to varying degrees.
Immunoaffinity technology is a new technology applied in the field of analysis in the 1990s, but there is no report on the use of immunoaffinity columns to purify sulfaquinoxaline drugs in the matrix, and there is no commercial IAC column for sale

Method used

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  • Method for extracting sulfabenzpyrazine from animal sample and special immune affinity sorbent thereof
  • Method for extracting sulfabenzpyrazine from animal sample and special immune affinity sorbent thereof
  • Method for extracting sulfabenzpyrazine from animal sample and special immune affinity sorbent thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1. Preparation of immunochromatographic column for purifying sulfaquinoxaline drugs

[0028] 1. Preparation of sulfaquinoxaline mouse monoclonal antibody

[0029] (1). Synthesis of immunogen

[0030] a. Synthesis of hapten

[0031] Such as figure 2 As shown, the sulfaquinoxaline (American Sigma company, catalog number: 967-80-6) and succinic anhydride are subjected to condensation reaction to obtain a hapten with a carboxyl functional group. The specific method is as follows: Weigh 100 mg of sulfaquinoxaline and 25mg of succinic anhydride was put into a round bottom flask, and anhydrous pyridine was added to it until it was completely dissolved. The reaction was heated and stirred at 80°C for 10 hours; after the reaction, the solvent was distilled off under reduced pressure. The residue was washed 5 times with acetone and ethyl acetate-n-hexane Alkane crystallizes to obtain the sulfaquinoxaline hapten.

[0032] b. Immunogen synthesis

[0033] The sulfaquinoxaline hapten...

Embodiment 2

[0059] Example 2. Preparation of a kit containing an immunochromatographic column coupled with a mouse monoclonal antibody and its purification effect on sulfaquinoxaline

[0060] 1. Preparation of kit containing immunochromatographic column

[0061] The kit is mainly composed of a box body, an immunochromatographic column (IAC column), a sulfaquinoxaline standard solution, a washing solution, an eluent, a preservation solution, and a sponge bracket. The sponge bracket is provided with holes and grooves. The grooves of the sponge bracket are equipped with reagent bottles respectively containing the sulfaquinoxaline drug standard solution, washing solution, eluent, and preservation solution, and the holes of the sponge bracket are equipped with IAC columns. The immunochromatographic column is the immunochromatographic column prepared in Example 1 that is coupled to the monoclonal antibody secreted by the monoclonal hybridoma cell line C-2-4CGMCC No.2571 of sulfaquinoxaline.

[0062] ...

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Abstract

The invention discloses a method for extracting sulfaquinoxaline from an animal sample and a special immunoaffinity absorbent thereof. The immunoaffinity absorbent for extracting the sulfaquinoxaline from the animal sample consists of a solid phase carrier and a sulfaquinoxaline monoclonal antibody which is coupled with the solid phase carrier; and the sulfaquinoxaline monoclonal antibody is obtained by taking a conjugate of sulfaquinoxaline semiantigen and carrier protein as immunogen. The purification method combines a chromatography to efficiently detect the content of a sulfaquinoxaline medicine, remedies the disadvantages that the direct assay of samples by a single immunoassay technology has small information amount, poor quantification accuracy, or low selectivity of physical and chemical methods and so on, and embodies the complementarity of an immunology technology and the prior physical and chemical technologies on an analysis mechanism.

Description

Technical field [0001] The invention relates to a method for extracting sulfaquinoxaline from animal samples and a special immunoaffinity adsorbent. Background technique [0002] With the development of life sciences, people have become more and more interested in the substances and changes in living organisms, and the analysis of biological samples has become a necessary means to explore and discover the mysteries of life. Due to the complex composition of biological samples, the low concentration of the analyte, and the small amount of most samples, this puts forward higher requirements on the selectivity and sensitivity of the analytical method. Immunoaffinity chromatography (IAC, immunoaffinity chromatography) is an analysis method that combines immune response and chromatography. Its high selectivity and high affinity undoubtedly simplify the analysis process. In the analysis of veterinary drug residues, the simplest and most effective application method of IAC is as a sam...

Claims

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Application Information

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IPC IPC(8): B01J20/286C07D241/44
Inventor 沈建忠张素霞史为民李炳玉万宇平曹兴元江海洋冯才伟李建成王战辉李存
Owner CHINA AGRI UNIV
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