Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications
A reagent board and liver cancer technology, applied in the field of biomedicine, can solve the problems of poor diagnostic specificity, missed detection and elevated levels of early liver cancer patients, and achieve the effect of easy operation
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[0032] Example 1 Preparation of the liver cancer detection reagent plate of the present invention
[0033] 1. Test materials and sources
[0034] 1 mouse anti-AFP monoclonal antibody
[0035]Mouse anti-AFP monoclonal antibody was purchased from National Laboratory of Tumor Cell Engineering, Xiamen University. Use an ultraviolet spectrophotometer to measure the protein concentration of not less than 2.0mg / ml; use SDS-PAGE electrophoresis to detect the purity is greater than 90%; use the ELISA indirect method to measure the antibody titer, when diluted to 1.0mg / ml, the titer is not lower than 1:256.
[0036] 2 Goat anti-mouse IgG
[0037] Goat anti-mouse IgG antibody was purchased from National Laboratory of Tumor Cell Engineering, Xiamen University. Use a UV spectrophotometer to determine the protein concentration is not lower than 4.0mg / ml; use SDS-PAGE electrophoresis to detect the purity is greater than 90%; use ELISA indirect method to determine the antibody titer, when...
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