Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications

A reagent board and liver cancer technology, applied in the field of biomedicine, can solve the problems of poor diagnostic specificity, missed detection and elevated levels of early liver cancer patients, and achieve the effect of easy operation

Inactive Publication Date: 2009-05-06
BEIJING WANTAI BIOLOGICAL PHARMACY ENTERPRISE
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term clinical studies have shown that AFP has certain limitations in the diagnosis of early liver cancer, as follows: 1. Poor diagnostic specificity: In addition to liver cancer, other diseases can also cause the increase of AFP in serum, especially about 20% - 40% of patients with chronic hepatitis and 20% - 50% of patients with liver cirrhosis also have elevated serum AFP, but these patients have not developed liver cancer, and the concentration of AFP in serum of chronic hepatitis or liver cirrhosis can also reach a very high level after acute deterioration levels, even exceeding 1000ng / ml
Second, the missed detection rate is high: in order to improve diagnostic specificity and differentiate benign and malignant liver diseases, the existing detection standards stipulate that AFP ≥ 400 ng / ml is specified as a specific indicator of liver cancer, but only 25.7% of patients with early liver cancer have AFP ≥ 400 ng / ml ml, so only detection of AFP results in missed detection of a large number of early liver cancer patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications
  • Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications
  • Reagent plate for rapidly detecting hepatocarcinoma, making method thereof and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of the liver cancer detection reagent plate of the present invention

[0033] 1. Test materials and sources

[0034] 1 mouse anti-AFP monoclonal antibody

[0035]Mouse anti-AFP monoclonal antibody was purchased from National Laboratory of Tumor Cell Engineering, Xiamen University. Use an ultraviolet spectrophotometer to measure the protein concentration of not less than 2.0mg / ml; use SDS-PAGE electrophoresis to detect the purity is greater than 90%; use the ELISA indirect method to measure the antibody titer, when diluted to 1.0mg / ml, the titer is not lower than 1:256.

[0036] 2 Goat anti-mouse IgG

[0037] Goat anti-mouse IgG antibody was purchased from National Laboratory of Tumor Cell Engineering, Xiamen University. Use a UV spectrophotometer to determine the protein concentration is not lower than 4.0mg / ml; use SDS-PAGE electrophoresis to detect the purity is greater than 90%; use ELISA indirect method to determine the antibody titer, when...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an agent plate for testing hepatocarcinoma and a preparation method and the application thereof. The agent plate is composed of a rigid plate and an agent strip attached to the rigid plate, and the agent strip is formed by sequentially and closely splicing absorbent filter paper, a pyroxylin membrane, a glass fibre membrane and a sample adsorption glass fibre membrane from top to bottom, wherein a gold-labeled anti-mouse AFP monoclonal antibody is absorbed by the glass fibre membrane; an agglutinin coated detection line is arranged at one end of the pyroxylin membrane, which is adjacent to the glass fibre membrane, and a goat anti mouse IgG coated contrast line is arranged at the other end which is adjacent to the absorbent filter paper; and the lower end of the sample adsorption glass fibre membrane is provided with a row of loading holes. The agent plate for testing hepatocarcinoma has the advantages that a fluorescence microscope, an Elisa tester and other expensive instruments are not needed, and the agent plate is much more suitable for field test; the agent plate is safer since radioactive isotopes, TMB and other harmful substances or chemical substances are not needed in testing processes; test results can be preserved for a long time; and the operation of the agent plate is easy and fast, and the agent plate can be operated by a single person.

Description

technical field [0001] The invention relates to a reagent plate for detecting diseases, in particular to a reagent plate capable of rapidly and accurately detecting liver cancer, a preparation method and application thereof, and belongs to the field of biomedicine. Background technique [0002] Since alpha-fetoprotein (AFP) has a relatively specific diagnostic value for hepatocellular carcinoma, it is widely used in clinical early detection of hepatocellular carcinoma. However, long-term clinical studies have shown that AFP has certain limitations in the diagnosis of early liver cancer, as follows: 1. Poor diagnostic specificity: In addition to liver cancer, other diseases can also cause the increase of AFP in serum, especially about 20% - 40% of patients with chronic hepatitis and 20% - 50% of patients with liver cirrhosis also have elevated serum AFP, but these patients have not developed liver cancer, and the concentration of AFP in serum of chronic hepatitis or liver cir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/577G01N33/574G01N33/558
Inventor 李益民
Owner BEIJING WANTAI BIOLOGICAL PHARMACY ENTERPRISE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products