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Method for preparing chook Marek's disease virus infection detection antigen

A Marek's disease, Marek's technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve high work efficiency, easy operation, and reduce production costs

Inactive Publication Date: 2009-04-15
张平仄 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, other preparation methods of MDV agar-amplified antigen have not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one: (truncated first, then divided and concentrated)

[0027] a. Remove the tested and customized screening membrane modules that allow the MDV A antigen to pass through effectively, such as hollow fiber ultrafiltration membrane modules marked with a molecular weight cut-off of 75,000, and roll-type ultrafiltration membrane modules with a molecular weight cut-off of 150,000. Seal the blocking plate, and install it in an appropriate ultrafiltration device after draining the maintenance fluid filled into it;

[0028]b. Open the liquid inlet valve, concentrated liquid return regulating valve, and liquid permeable valve to the maximum flux position, turn on the booster pump, rinse with clean water at room temperature (20-30°C) for 20-30 minutes, and adjust through the concentrated liquid return flow The operating pressure of the valve control is within the working range (0.02~0.1Mpa);

[0029] C, will contain the raw material liquid of MDV A antigen, as the sup...

Embodiment 2

[0032] Embodiment two: (remove small and concentrate earlier, then remove large purification, concentrate again)

[0033] With reference to the procedures, parameters and process conditions of Example 1, first use the custom-made maximum molecular weight cut-off membrane module capable of effectively retaining the MDV A antigen to carry out ultrafiltration concentration and removal of small molecular impurities on the thin raw material solution containing the MDV A antigen. When it is concentrated to a specified multiple or the permeate is reduced to a very low flow rate, the permeate is discarded and the retentate is collected. The residue in the equipment is also incorporated into the retentate after being circulated and washed with purified water (or physiological saline, or PBS buffer solution). Then, add not less than 3 times the volume of clean water or physiological salt or PBS buffer to the liquid for mixing, and use the ultrafiltration with the minimum molecular weigh...

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PUM

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Abstract

The invention discloses a preparation method of AGP detection antigen used for diagnosing Marek's virus infection, relating to the poultry disease detection reagent field. The method comprises the steps as follows: firstly, the separating effect experimental evaluation is carried out on the target virus antigen, so that a permeation-typed filter membrane element and a retention-typed filter membrane element are screened out from ultrafiltration membrane elements with different molecular weight cutoff; secondly, the permeation-typed filter membrane element and the retention-typed filter membrane element which are screened out are respectively used to purify and concentrate the liquid containing the target virus antigen; finally, the purified and concentrated solution is detected and quantified, so that the Marek's virus infection AGP detection antigen is prepared; and no reagent is added in the process of ultrafiltration treatment, therefore, not only neither secondary pollution nor contamination is produced in the production process, but also the emission liquid is actually further purified, so as to reduce the pollution to the environment and improve resource utilization, thereby really achieving the purpose of transforming trash into treasure, and the preparation method also has the advantages of no phase state change, mild conditions, simple operation, low cost and high recovery rate, etc.

Description

Technical field: [0001] The invention relates to the field of poultry disease detection reagents, in particular to a preparation method of chicken Marek's virus infection detection antigen. Background technique: [0002] Marek's disease (MD) is the world's first tumorigenic and immunosuppressive infectious disease that has been controlled by vaccines and has attracted much attention from the veterinary, medical and biological circles. Its pathogen is Marek's herpes virus (MDV). MDV is a cell-associated virus, which has three serotypes, ranging from mild to highly virulent tumorigenic strains and their attenuated strains, all of which are classified as serotype 1 MDV, and the naturally non-tumorogenic virus strains are serotype 2 , turkey herpesvirus (HVT) belongs to serotype 3; among the three serotypes, only the virulent strain in serotype 1 is tumorigenic or pathogenic to chickens. Because the disease seriously damages the immune system of chickens, in addition to causing...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/569
Inventor 张平仄张训海
Owner 张平仄
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