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Novel uses of Salicornia bigelovii and root extract thereof

A technology of samphire and extract, applied in the field of samphire and its root extracts, can solve the problems of affecting the repair effect, secondary eutrophication pollution, red tide and the like

Inactive Publication Date: 2009-04-15
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing studies have shown that due to problems such as seaweed growth seasonality and fouling organisms (Blindow et al. 2002 Seasonal changes of mechanisms maintaining clear water in a shallow lake with abundant Chara vegetation. Aquatic Botany 72: 315-334; Gross et al. 2007 Searching for allelopathic effects of submerged macrophytes on phytoplankton-state of the art and open questions. In: Gulati RD, Lammens E, Pauw ND, Donk EV(eds) Developments in Hydrobiology, Shallow Lakes in a Changing; Zhu et al.2007 Can Dreissen Attachment and Biodeposition Affect Submerged Macrophyte Growth? Journal of Aquatic Plant Management 45:71-76) seriously affects the restoration, and the overgrowth of algae and the re-release of nutrients after death cause secondary eutrophication pollution, which in turn leads to red tides Occurs more frequently, large seaweeds such as asparagus and Gracilaria cannot fundamentally solve the serious eutrophication problem in coastal waters

Method used

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  • Novel uses of Salicornia bigelovii and root extract thereof
  • Novel uses of Salicornia bigelovii and root extract thereof
  • Novel uses of Salicornia bigelovii and root extract thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, North American salicornia to the restoration effect of eutrophic seawater

[0029] 1. Growth of Salicornia saponica in different eutrophic seawater

[0030] After cultured for 45 days, the seedlings with stems about 10 cm long and with the same growth were taken and placed in a blue polyethylene plastic water tank of 34.5 cm × 24.5 cm × 12 cm. The transparent plastic plate was used as the planting plate. Twelve planting holes with a diameter of 2cm were opened at a distance of 6cm×6cm, and 12 plants were placed in each box. The experimental water samples are shown in Table 1. The volume of each water sample is 10L, 24h aeration and water circulation, the photoperiod is 12h, and the average light intensity is 130μmol m -2 the s -1 , air temperature 22 / 30°C (22°C is the temperature in the dark, 30°C is the temperature in the light). For each group of treatments, 5 parallel samples were set up and repeated 3 times, of which 2 parallel samples were used as...

Embodiment 2

[0044] Embodiment 2, the suppression of the growth of Sclerotinia costarum by Salicornia saponica in North America

[0045] Take the North American salicornia plant that has been cultivated for 45 days, and place it in a transparent glass water tank with a specification of L×W×H=30cm×20cm×30cm, and use a transparent plastic tank of L×W×H=34.5cm×24.5cm×0.12cm The plate is a planting plate, on which 12 planting holes with a diameter of 2 cm are opened with a spacing of 6 cm × 6 cm, and 12 plants are placed in each case, with a total fresh weight of 70 g. Each treatment was inoculated with Scutellaria costatum in exponential growth phase at an initial inoculation density of 3×10 5 cells / ml, a total of 0.25ml was added. The control group that was not inoculated with Salicornia sativa was used as the control group, and 3 replicates were set up for each treatment. The water samples were collected from Yundang Lake (YDH) 3# station, the volume of each box of water samples was 15L, ...

Embodiment 3

[0056] Embodiment 3, the inhibition of the root system water extract of Salicornia chinensis to the growth of Sclerotinia costatum

[0057] 1. Preparation of Root Water Extract

[0058] Get fresh weight and be 60g North American Salicornia root system (North American Salicornia seedlings are purchased from Jiangsu Jinglong Ocean Industry Development Co., Ltd.), use 100ml sterile seawater as the extract, grind into a homogenate, extract in an ultrasonic oscillator for 1h, Then centrifuge at room temperature 10000g for 10min, repeat 3 times, discard the residue, combine the supernatant and filter through Whatman GF / F, the filtrate is stored at 4 ° C, and is used as a mother liquor The fresh weight of the Salicornia root system is calculated as: 600g North American Salicornia root fresh weight / per liter solution).

[0059] 2. Inoculation and cultivation of Skeletalum costatum

[0060] Dilute the above mother liquor with sterile seawater to obtain a series of concentration gradi...

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Abstract

The invention discloses new usages of Salicornia bigelovii Torr and the extractive of the root system thereof, including the applications of the Salicornia bigelovii Torr and the extractive of the root system thereof in restoring eutrophication water body and in inhibiting the growth of Skeletonema costatus as well as red tide. As shown by test result, the Salicornia bigelovii Torr can absorb a great quantity of nitrogen and phosphorus nutrient salt and can effectively remove various inorganic nutrient salts in water; besides, the Salicornia bigelovii Torr can dramatically reduce the concentration of chlorophyll and the amount of Skeletonema costatus in water; and moreover, the extractive of the root system can considerably inhibit the growth of the Skeletonema costatus and have a stronger inhibiting effect with the increase of concentration; when the concentration reaches 10.4g / l, the inhibiting ratio reaches 50 percent; and when the concentration reaches 30g / l, the inhibiting ratio reaches the maximum.

Description

technical field [0001] The invention relates to a new application of salicornia saponica and its root system extract. Background technique [0002] With the rapid development of the global economy, problems such as population expansion, food shortage, environmental degradation, and land resource depletion have become increasingly prominent. The ocean, especially the coastal waters, is of great significance to human beings. However, with the rapid increase of population in coastal areas and the rapid development of industry and agriculture, serious organic pollution and eutrophication have occurred in inner bays, estuaries and coastal waters. The scale and frequency of harmful red tides in China are on the rise. How to effectively prevent and control red tide has become a major problem to be solved urgently. [0003] Since the 1990s, phytoremediation technology has gradually become a research hotspot as a new technology, especially the phytoremediation effect of plants provi...

Claims

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Application Information

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IPC IPC(8): C02F3/32
Inventor 李银心黄凌风姜丹林施泉张语克
Owner INST OF BOTANY CHINESE ACAD OF SCI
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