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Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection

A technology of red blood cell membrane and kit, applied in the biological field, can solve the problems of difficult acquisition, short storage period, blank antibody screening, etc., and achieve the effect of simple detection method, favorable preservation, and easy automation

Inactive Publication Date: 2009-03-18
长春博德生物技术有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibody screening for blood donors is even blank, and there is a great potential safety hazard
[0010] At present, the detection cells used in clinical irregular antibody screening are the screening cells preserved in the red blood cell preservation solution. The screening cells are obtained by screening the antigen spectrum from the O-type blood donors. It is not easy to obtain and has a short storage period. Commonly used, so often a set of reagents will cause hemolysis after a small number of specimens are tested, and it cannot be used continuously, which is a great waste

Method used

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  • Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection
  • Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection

Examples

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preparation example Construction

[0026] Preparation of Erythrocyte Membrane Antigen Magnetic Beads:

[0027] 1. Preparation of erythrocyte antigens:

[0028] (1) Take fresh blood cells, centrifuge to remove the supernatant, and take the packed blood cells for the next step;

[0029] (2) Add SDS to the packed blood cells, and then place at room temperature;

[0030] (3) centrifuge to remove supernatant, leave sediment and add distilled water to the sediment;

[0031] 2. Preparation of magnetic particles:

[0032] (1) FeCl 2 and FeCl 3 Add to distilled water to dissolve;

[0033] (2) under the condition of vigorous stirring, sodium hydroxide is added to the above-mentioned metal salt solution, and it is hydrolyzed into metal composite oxide, thereby obtaining ferric oxide particle solution;

[0034] 3. Cell membrane antigen-coated magnetic particles

[0035] Add the red blood cell membrane antigen obtained in step 2.1 to the ferric tetroxide particle solution obtained in step 2.2, then add polyethylene g...

Embodiment 1

[0041] Example 1 ABO system blood typing test

[0042] A red blood cell membrane antigen magnetic beads and B red blood cell membrane antigen magnetic beads are prepared by using known type A red blood cells and type B red blood cells as in the specific embodiment. Since the red blood cell membrane antigen magnetic beads are coated with A antigen and B antigen, they can replace fresh red blood cells for ABO system blood typing detection. Type A human serum contains anti-B antibodies, type B human serum contains anti-A antibodies, type AB human serum does not contain antibodies, and type O human serum contains anti-AB antibodies. Most of the antibodies of the ABO blood group system are naturally occurring antibodies, which are IgM antibodies. The red blood cell membrane antigen magnetic beads can specifically immunoreact with the corresponding IgM antibodies in the serum to form agglutination visible to the naked eye. The blood type is determined according to the pattern after...

Embodiment 2

[0046] Example 2 Screening of erythrocyte irregular antibodies

[0047] Red blood cells are detected with antibodies of known specificity, and the antigenic profile of the screened cells is determined as follows:

[0048]

[0049] The above-mentioned screening cells are prepared by screening cell membrane magnetic beads as in a specific embodiment. The surface of the screening cell membrane antigen magnetic beads has all the antigens on the fresh screening cells, which can be used for clinical screening of IgG antibodies. IgG antibodies are generally produced due to alloimmunization such as blood transfusion and pregnancy. It is a 7S globulin with a small molecular weight and a molecular length of only 250A 0, after binding with the corresponding antigen of erythrocytes, it can only bind with erythrocytes in saline medium without visible agglutination reaction, and the sensitized cells must be agglutinated by adding anti-human globulin reagent, so as to detect such antibod...

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Abstract

A red cell membrane antigen magnetic bead kit and an application in blood type antibody test belong to the biological technical field, for using red cell membrane antigen magnetic beads to prepare a kit and provide the application in blood type antibody test. The kit comprises red cell membrane antigen magnetic beads, a U-shaped reaction plate of 96 holes and a red cell membrane antigen magnetic bead diluent. The invention has the advantages that the red cell membrane antigen magnetic beads have long storage period, thus is beneficial for the storage of red cell rare antigens.

Description

Technical field: [0001] The present invention belongs to the field of biotechnology. Background technique: [0002] In 1900, Landsteiner discovered the ABO blood group antigen of human red blood cells. This epoch-making achievement opened the world's in-depth discussion of clinical blood transfusion and laid the foundation for today's clinical safe blood transfusion. [0003] Blood group serological testing before clinical blood transfusion can be summarized into three categories: 1. blood group testing; 2. antibody screening and identification; 3. cross-matching blood. Blood type test: check to determine the red blood cell blood type of the recipient and blood donor, the most important is the ABO blood type and the Rh blood type. The British Pharmacopoeia particularly emphasizes that the detection of ABO blood type not only needs to detect red blood cell surface antigens, but also must detect antibodies in serum (or plasma), that is, positive and negative typing. That is ...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/551
Inventor 李勇童军张宇杨文冲段生宝孔维臣景维东周墅李东孙玉昌马学严
Owner 长春博德生物技术有限责任公司
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