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Thyrotropin chemiluminescence immune analysis determination reagent kit and preparing method thereof

A chemiluminescence immunity, thyrotropin technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, biological testing, etc., can solve the problems of large loss of activity, short half-life, large amount of antibody, etc. The clinical compliance rate is improved, the linearity is good, and the sensitivity is ensured

Inactive Publication Date: 2009-03-04
北京科美东雅生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have unavoidable deficiencies: environmental pollution by radioactive elements and short half-life limit the wide application of RIA and IRMA; the sensitivity of enzyme immunosorbent assay is not good enough to meet clinical requirements; direct chemiluminescence immunoassay is Short luminescence time requires high-precision automatic analysis system; electrochemiluminescence detection and time-resolved fluorescence detection require expensive and complex instruments and equipment
Simply using physical adsorption to coat the monoclonal antibody on the solid phase carrier (polystyrene plate) will have the disadvantages of large amount of antibody and large loss of activity, and the use of FITC-anti-FITC system can avoid such shortcomings

Method used

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  • Thyrotropin chemiluminescence immune analysis determination reagent kit and preparing method thereof
  • Thyrotropin chemiluminescence immune analysis determination reagent kit and preparing method thereof
  • Thyrotropin chemiluminescence immune analysis determination reagent kit and preparing method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Preparation of the TSH chemiluminescence immunoassay assay kit of the present invention

[0028] 1. Preparation of TSH calibrator

[0029] Dilute the pure TSH product into a calibrator with hormone-free human serum, and distribute 0, 0.1, 0.5, 2.5, 10, 40 μ IU / mL into 6 bottles in total.

[0030] 2. Preparation of anti-FITC antibody-coated microplates

[0031] 1) coated

[0032] Prepared by mixing 1000mL of 0.046M citric acid buffer solution with a pH value of 4.6 (contains 4.44g of citric acid and 7.3g of trisodium citrate deionized aqueous solution to make a buffer solution), mixed with an appropriate concentration of anti-FITC monoclonal antibody Coating solution, and load it on the microwell plate;

[0033] 2) washing the above-mentioned microwell plate with PBST washing solution; and

[0034] 3) closed

[0035] Prepare a blocking solution, the blocking solution contains 0.1M Tris, 150mM sodium chloride, 1% BSA and 0.1% biological preservative, the pH...

Embodiment 2

[0068] Embodiment 2 The usage method of kit of the present invention

[0069] 1. Sample pretreatment

[0070] The fasting morning serum samples were taken from people, centrifuged at 3000rpm for 5min, and the upper serum was taken for analysis.

[0071] 2. Detection method

[0072] Follow the steps below to use this kit:

[0073] 1) Preparation of washing buffer: dilute the washing buffer provided in the kit with distilled water 10 times;

[0074] 2) Take the calibrators, antibody-coated microplates, FITC markers and enzyme markers out of the refrigerator at 4°C, place them for 15 minutes, and equilibrate to room temperature;

[0075] 3) Add 50 μL of FITC-labeled TSH monoclonal antibody to the reaction wells, then add the sample to be tested and 25 μL of 0, 0.1, 0.5, 2.5, 10, 40 μ IU / mL for each well, and finally add horseradish 50 μL of peroxidase-labeled TSH monoclonal antibody solution, shake well, and react at 37°C for 45 minutes;

[0076] 4) Discard the solution in t...

Embodiment 3

[0081] Embodiment 3 The methodological test of the kit of the present invention

[0082] The test kit prepared in Example 1 is tested according to conventional manufacturing and testing procedures in the art. The present invention has tested the precision, accuracy, sensitivity, specificity and stability of the test kit, and the results are as follows:

[0083] 1. Determination of kit precision

[0084] Three batches of the kits prepared in Example 1 were used for precision experiments, and three different concentrations of serum, namely low, medium, and high, were measured respectively. 10 wells were measured in parallel, and repeated 3 times to obtain the intra-assay and multiple of each analysis. Inter-assay variability (Table 1). The intra-assay and inter-assay coefficients of variation were both less than 10%.

[0085] Table 1 Inspection of method precision

[0086]

[0087] 2. Determination of kit accuracy

[0088] Accuracy refers to how close a measurement is to ...

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Abstract

The invention provides a thyrotropin (TSH) chemiluminescent immunoassay quantitative detection kit and a preparation method thereof. The invention has the advantages that the reaction pattern of the double antibody sandwich method is adopted, the chemiluminescent technology combining with the technology of fluorescin isothiocyanate-anti-fluorescein isothiocyanate is effectively utilized, horse radish peroxidase is adopted to be as the marker enzyme to quantitatively detect the content of TSH in the human serum sample, the detection sensitivity is guaranteed, and the raw materials are greatly saved. The kit of the invention has high sensitivity, good repeatability, short reaction time, good stability and long reagent validity, and can provide the experimental basis timely for the clinical diagnosis and the treatment of thyroid disorders.

Description

technical field [0001] The invention relates to the field of immune detection and analysis, in particular to a thyrotropin assay kit and a preparation method thereof. The kit of the invention combines fluorescein isothiocyanate (FITC)-anti-fluorescein isothiocyanate technology and chemiluminescence immunoassay technology. Background technique [0002] Thyroid-stimulating hormone (TSH) is a glycoprotein hormone secreted by the anterior lobe of the pituitary gland, and its concentration is controlled by thyrotropin-releasing hormone secreted by the pituitary gland and thyroxine T in the blood. 4 and T 3 Impact. It can regulate the function of thyroid cells, affect thyroid hormone synthesis, glucose oxidation and protein synthesis. When thyroid function changes, TSH levels fluctuate significantly. Determining the concentration of TSH in serum is the first choice index to judge whether the thyroid function is normal, and it is also an important parameter to study the feedbac...

Claims

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Application Information

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IPC IPC(8): G01N33/78G01N33/577G01N33/543G01N21/76
Inventor 辛天兵应希堂宋胜利胡国茂唐宝军于尚永
Owner 北京科美东雅生物技术有限公司
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