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Chemiluminescence immune analysis determination reagent kit for detecting Toxoplasma Gondi IgM antibody

A chemiluminescence and reagent kit technology, applied in the field of medical immunology and in vitro diagnosis, can solve the problems of inconvenient operation of etiology experiments, pollution hazards of radioactive waste, and short validity period of radioimmunization reagents, so as to facilitate the monitoring of the production process and avoid immunological Large loss of activity, avoiding toxic or carcinogenic effects

Inactive Publication Date: 2009-03-04
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention solves the problems of inconvenient operation, low sensitivity, short validity period of radioimmunization reagents, pollution hazards of radioactive waste, interference of enzyme immunity reagents by various factors, and most of the substrates being poisonous in current clinical etiology tests.

Method used

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  • Chemiluminescence immune analysis determination reagent kit for detecting Toxoplasma Gondi IgM antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Toxoplasma gondii IgM antibody chemiluminescence immunoassay assay kit of the present invention

[0029] 1. Preparation of negative control and positive control

[0030] 1. Preparation of negative control

[0031] Collect more than 6 normal human sera that are negative for Toxoplasma gondii IgM antibody, HIV (1+2) antibody, HCV antibody, TP antibody, and HBsAg negative by ELISA, filter and sterilize, subpackage, and store at low temperature.

[0032] 2. Preparation of positive control

[0033] Collect more than 6 sera from patients who are positive for Toxoplasma gondii IgM antibody and negative for HIV (1+2) antibody, HCV antibody, TP antibody, and HBsAg by ELISA, filter and sterilize, subpackage, and store at low temperature.

[0034] 2. Preparation of anti-FITC coated microplates

[0035] 1. Coating

[0036] Preparation of coating solution: 5.8g Na 2 HPO 4 12H 2 O and 0.59 g NaH 2 PO 4 2H 2 O was dissolved in double distilled water ...

Embodiment 2

[0084] Example 2 Preparation of Toxoplasma gondii IgM antibody chemiluminescence immunoassay assay kit of the present invention

[0085] Except that the magnetic particles were used as the solid phase carrier, and the anti-FITC was coupled to the surface of the magnetic particles by the glutaraldehyde method, the rest were prepared in the same manner as in Example 1 to prepare the Toxoplasma gondii IgM antibody chemiluminescent immunoassay assay kit.

Embodiment 3

[0086] Embodiment 3 The usage method of the kit of the present invention

[0087] The specific operations of the Toxoplasma gondii IgM antibody chemiluminescence immunoassay assay kit prepared in the above embodiment 1 are as follows:

[0088] 1) Take out the Toxoplasma gondii IgM assay kit (chemiluminescence method) from the refrigerator at 2-8°C, and equilibrate at room temperature for 30 minutes;

[0089] 2) Pre-dilute the sample to be tested with physiological saline at a ratio of 1:1000;

[0090] 3) For each experiment, 3 wells of TOX IgM negative control and 2 wells of positive control were set. Add 50 μl of negative control, positive control and pre-diluted sample to be tested, then add 50 μl of FITC marker to each well, shake and mix well, and incubate at 37°C for 30 minutes;

[0091] 4) Wash 5 times with diluted washing solution, fill each well with washing solution, soak for 10 seconds each time, and finally pat dry on clean absorbent paper;

[0092] 5) Add 100 μl...

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Abstract

The invention discloses a toxoplasma gondii IgM antibody detection kit combined with the FITC-anti-FITC indirect coating technology and the chemiluminescent immunoassay technology, and a preparation method thereof. The kit of the invention is composed of a negative control, a positive control, solid-phase vectors for anti-FITC antibodies, anti-human Mu-chain monoclonal antibodies of FITC markers, toxoplasma gondii antigens which are marked by horse radish peroxidase, chemiluminescent substrates and concentrated washing solutions. The kit of the invention can be used as the aided detection index for prenatal prepotency diagnosis, and has vital significances for improving the birth population quality and doing the family planning and the prepotency well.

Description

technical field [0001] The invention relates to the technical field of medical immunology and in vitro diagnosis. Specifically, the invention provides a FITC-anti-FITC antibody indirect coating technology combined with a chemiluminescent immunoassay technology to detect Toxoplasma gondii IgM antibody assay kit and a preparation method thereof. Background technique [0002] Toxoplasma gondii is an intestinal coccidia of cats. It was discovered by French scholars Nicolle and Manceaux in the spleen mononuclear cells of Ctenodactylus gondii. Toxoplasma gondii. Toxoplasmosis, also known as toxoplasmosis, is a zoonotic parasitic disease caused by Toxoplasma gondii. According to literature reports, Toxoplasma gondii can infect a variety of mammals, and the infection rate of livestock can reach 10-50%. There are about 1 billion people infected with Toxoplasma gondii in the world, and the average infection rate of our population is 5.16%. Toxoplasma gondii is one of the five major...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543G01N21/76
Inventor 彭京胜应希堂宋胜利胡国茂郑金来唐宝军
Owner CHEMCLIN DIAGNOSTICS CO LTD
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