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Cudrania tricuspidata extract, preparation and application thereof

A technology of extract and Zhemu, which is applied in the field of natural medicine and traditional Chinese medicine extract, can solve the problems of insufficient purification process of Zhemu extract, great influence on the quality of Zhemu extract, and unsound process indicators, etc., and achieve remarkable and broad anti-tumor effect Market application prospects, controllable effects of active ingredients

Active Publication Date: 2012-03-21
SHANGHAI LEIYUNSHANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese patent 03114766.6 discloses a solid preparation containing Zhemu extract, which mentions Zhemu extract, but the purification process of the method for preparing Zhemu extract in this patent is not perfect, and the process indicators are not perfect. The quality of the obtained Zhemu extract is It is greatly affected by the initial medicinal materials, and the controllability is relatively weak

Method used

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  • Cudrania tricuspidata extract, preparation and application thereof
  • Cudrania tricuspidata extract, preparation and application thereof
  • Cudrania tricuspidata extract, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Quercetin-7-O-β-D-glucoside in Zhemu

[0048] Isolation and Identification of Kaempferol-7-O-β-D-glucoside

[0049] Take the concentrated solution of Zhemu extract extracted with water or dilute alcohol, pass directly through a macroporous resin column that has been treated with water phase, and after fully eluted with water, gradient elution with 30% to 95% ethanol solution, the collected 30% The ~50% ethanol fraction, after concentration, is put on a silica gel column, and is eluted with a gradient of ethyl acetate-methanol (0-30% methanol), and the eluent is detected by TLC method. The development conditions of silica gel TLC detection : Ethyl acetate-acetone-formic acid-water (5:3:0.5:0.5), color development conditions: spray AlCl3 ethanol solution, evaporate the thin plate and check under 365nm fluorescence to see whether the components to be separated are related to quercetin-7 -O-β-D-glucoside or kaempferol-7-O-β-D-glucoside at the same position in the...

Embodiment 2—5

[0058] The preparation of embodiment 2-5 eucalyptus extract (extra-macroporous resin chromatography column)

[0059] Extraction process: 100kg Zhe wood small pieces, use 5-10 times the weight of the original medicinal materials in 0%-70% alcohol aqueous solution, reflux extraction twice, each time for 1-5 hours, combine the extracts, concentrate under reduced pressure until the medicinal liquid is alcohol-free taste.

[0060] Purification process: pass the concentrated solution through a neutral macroporous resin column (HPD-100, Baoen Chemical), then elute with 3-6 times the column volume of pure water, and use 2-4 times the column volume of 20 %, 40%, 60%, and 80% ethanol were eluted, and the 40-60% ethanol eluate was collected and concentrated to dryness.

[0061] Selection of extraction process parameters and obtained results:

[0062]

[0063] Ingredient 1: Kaempferol-7-O-β-D-glucoside;

[0064] Ingredient 2: Quercetin-7-O-β-D-glucoside

Embodiment 6

[0065] The preparation of embodiment 6 eucalyptus extract (excessive macroporous resin and gel chromatography column)

[0066] Get the 40-60% ethanol fraction partial concentrate obtained in Example 3, pass through a chromatographic column equipped with gel (Sephadex LH-20, Pharmacia), and wash with 50% ethanol of 4-8 times the column volume , and then eluted with 95% ethanol of 4-8 times the column volume, collected 95% ethanol fractions, concentrated under reduced pressure, and dried in vacuo to obtain 100 g of extract powder, wherein the total flavonoid content was 35%, kaempferol-7- O-β-D-glucoside 9%, quercetin-7-O-β-D-glucoside 8%, kaempferol-7-O-β-D-glucoside with quercetin-7-O- The weight ratio of β-D-glucoside is 1:0.88.

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Abstract

The invention relates to the technical field of natural medicaments and traditional Chinese medicine extracts, and discloses a tsugeki extract. The weight ratio of kaempferol-7-O-beta-D-glucoside and quercetin-7-O-beta-D-glucoside in the extract is 1: (0.6 to 1.1); on the basis of the weight of the extract, the weight percentage of total flavone is 1.5 to 50 percent, wherein the weight percentageof the kaempferol-7-O-beta-D-glucoside is 0.3 to 20 percent; and the weight percentage of the quercetin-7-O-beta-D-glucoside is 0.2 to 15 percent. The invention further discloses a preparation process and the application for the tsugeki extract. The preparation method for the tsugeki extract has the advantages of perfect process, perfect process index, controllable prepared tsugeki extract and active components of various preparations, as well as remarkable anti-tumor effect, and has broad prospect for market application.

Description

technical field [0001] The invention relates to the technical field of natural medicine and traditional Chinese medicine extract. Background technique [0002] Zhemu is a deciduous shrub or small tree of the genus Cudrania tricuspidata (Carr.) Bun in the Moraceae family. Its function is to clear away heat and cool blood, and it is used for digestive tract tumors in modern folk. The main chemical components are flavonoids, xanthones, lignans, coumarins and polysaccharides. From the 1970s to the 1990s, domestic and foreign scholars (China, Japan and South Korea) conducted research on the anti-cancer effects of Zhemu, and basically confirmed that Flavonoids are anti-cancer and anti-cancer effective components of Zhemu. Polysaccharides participate in immune regulation and have a synergistic effect on anti-cancer. In addition, regarding kaempferol, quercetin and their glycoside compounds, it is generally believed internationally that they have physiological activities such as an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K36/60A61K9/00A61K9/16A61K9/19A61K9/20A61K9/48A61P35/00C07H1/08
Inventor 张国明谢家骏
Owner SHANGHAI LEIYUNSHANG PHARMA
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