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Apparatus for ordered adhering various cells to setting position of same substrate and adhesion method

A technology for setting positions and substrates, applied in biochemical equipment and methods, immobilized enzymes, determination/inspection of microorganisms, etc., which can solve complex operations, cell movement that cannot control adhesion well, and cell patterns that cannot interact with each other. role, etc.

Inactive Publication Date: 2009-02-11
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the complex operation of the prior art when adhering various cells to the same substrate surface, and the movement of the adhered cells cannot be well controlled, so that the fixed cell pattern is destroyed or cannot interact at all. , thereby providing a device and an adhesion method for orderly adhering various cells to a set position on the same substrate, which has a simple structure and is easy to operate, can effectively control the distance between different types of cells, and can control the distance between different types of cells. The movement between cells is convenient for studying the interaction between different kinds of cells

Method used

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  • Apparatus for ordered adhering various cells to setting position of same substrate and adhesion method
  • Apparatus for ordered adhering various cells to setting position of same substrate and adhesion method
  • Apparatus for ordered adhering various cells to setting position of same substrate and adhesion method

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Experimental program
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Effect test

Embodiment 1

[0075] Please refer to Figure 2-1, Figure 2-2, Figure 2-3 and Figure 2-4:

[0076] 1) Evaporate a 10nm (2-10nm) titanium adhesion layer on the surface of a clean glass slide 1 (thickness 0.15mm), and then vapor-deposit a 40nm (20-50nm) titanium adhesion layer on the surface Thick gold layer, making substrate 1;

[0077] Cut the prepared substrate 1 into 2x2cm 2 Soak the small pieces in an aqueous solution of ethanol (concentration: 50% to 100%), then blow dry with nitrogen for subsequent use;

[0078] 2) using photolithography technology to prepare a pattern with at least one set of convex strip microstructure units on a silicon wafer, the convex strip microstructure units comprising:

[0079] The first convex strip, the second convex strip, the third convex strip, the fourth convex strip, the fifth convex strip and the sixth convex strip with a pitch of 50 μm arranged in sequence from left to right; The middle sections of the first convex strip, the second convex strip, th...

Embodiment 2

[0091] 1) Evaporate a 10nm (2-10nm) titanium adhesion layer on the surface of a clean glass slide 1 (thickness 0.15mm), and then vapor-deposit 20nm (20-50nm is acceptable) on the titanium adhesion layer Thick gold layer, making substrate 1;

[0092] Cut the prepared substrate 1 into 2 x 2cm 2 Soak the small pieces in an aqueous solution of ethanol (concentration: 50% to 100%), then blow dry with nitrogen for subsequent use;

[0093] 2) using photolithography technology to prepare a pattern with at least one set of convex strip microstructure units on a silicon wafer, the convex strip microstructure units comprising:

[0094] The first convex strip, the second convex strip, the third convex strip, the fourth convex strip, the fifth convex strip and the sixth convex strip with a pitch of 100 μm arranged in sequence from left to right; The middle sections of the first convex strip, the second convex strip, the third convex strip, the fourth convex strip, the fifth convex strip ...

Embodiment 3

[0106] Immerse the substrate adhered with various cells obtained in the embodiment into ordinary cell culture medium (fetal calf serum concentration 10%); after the two kinds of cells are orderly fixed on the surface of the substrate, the positive electrode of the power supply with a voltage of 1.0-1.4V Connected to the cell culture solution, the negative electrode is connected to the gold surface of the base of the cell adhesion, and electrolysis is carried out. Since the thiol can be desorbed from the gold surface after electrification, and the poly(L-lysine)-polyethylene Diol-poly(L-lysine) will not desorb from the gold surface, so as to control one kind of cell to not move, and another kind of cell to crawl freely, so that one kind of cell can be studied at different distances from another kind of cell What is the difference in the impact of the situation; the duration of electrolysis is 30s.

[0107] In addition, the convex strips of the convex strip type microstructure u...

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Abstract

The invention relates to a device and an absorption method for adhering a plurality of types of cells on a set position of a same substrate, the device comprises: the substrate with the upper surface which is evaporated with a titanium / chromium adhesion layer and a metal layer and a polydimethylsiloxane seal with a hydrophilic surface and the lower surface which comprises at least one group of micro-groove units; the lower surface of the seal is adhered on the metal layer to form a sealed pipeline by reversible combination; FN is introduced to the second and the fifth grooves to form extracellular matrix proteins within 10min; ethanol water solution of block copolymer of poly (L-lysine)-polyethylene glycol-poly (L-lysine) is introduced to the first and the third grooves to form absorption layers; EG6 is introduced to the fourth and the sixth grooves to form inert mono-molecular film layers in and around the fourth and the sixth grooves. The device and the absorption method can lead a plurality of types of cells to be adjustably and orderly adhered on the same substrate, thereby understanding the effects of a drug to the different types of the cells, screening the drugs and providing a new route for founding the drugs and carrying out the analysis of toxic substances.

Description

technical field [0001] The present invention relates to a device and an adhesion method for orderly adhering various cells to the same substrate, in particular to a device and an adhesion method for adhering various cells to the same substrate in an orderly and controllable manner . Background technique [0002] In the basic research fields of cell biology and tissue biology, as well as the applied research of biomedical engineering and drug detection based on the interaction between cells, it is often necessary to immobilize two or more kinds of cells on the same surface. Recently, some methods of related methods have been reported. [0003] For example in literature 1: Chiu, D.T.; Jeon, N.L.; Huang, S.; Kane, R.S.; Wargo, C.J.; , (6), 2408-2413, the Whitesides experimental group disclosed a method of immobilizing various cells on the same surface using a three-dimensional microfluidic channel, and can form a complex pattern composed of various cells. This method first p...

Claims

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Application Information

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IPC IPC(8): C12N11/00C12Q1/04
Inventor 李勇陈振玲蒋兴宇
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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