Double membrane tissue patching material and preparation method thereof

Abstract

The invention discloses a double-layer membranous tissue repair material and a preparation method thereof, wherein, a cell-free membranous biological derivative material is used as a surface layer, and a fibroblast is compounded in the interior of a biological support material to form a substrate, and then the surface layer and the substrate are combined in a chimeric way to form the double-layer membranous tissue repair material; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as prevent the infection of the surface of wound, thus being beneficial to epitheliosis and epithelial growth; the substrate can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; compared with the existing products, the tissue repair material has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing hyperplasia of scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the invention has wide material resources and simple production method; the double-layer membranous tissue repair material prepared is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to a double-layer membranous tissue repair material and a preparation method thereof. Background technique [0002] Skin is an important tissue organ that covers and protects the body surface. Defects and abnormalities of the skin are often caused by inflammation, ulcers, trauma, burns, postoperative tumors, and congenital deformities. For exposed skin wounds, gauze is traditionally used the most. Gauze is mostly processed from cotton, burlap and linen, and is an inert material that does not directly promote wound healing. With the in-depth research on wound healing, it is realized that not only the wound surface must be covered, but also the wound must be helped to heal. Biomaterials composed of extracellular matrix are one of the ideal products, and their main components include collagen, non-collagenous glycoproteins, elastic fibers, glycosa...

AI Technical Summary

Problems solved by technology

[0004] At present, the commercialized double-layer membranous biomaterial has Integra (produced by Integra LifeSciences), which is an artificial skin with a double-layer matrix. The cortex is composed of a silicone membrane, which can control the evaporation of wound moisture. During the wound healing process, the dermis will combine with the wound. The silicone membrane of the epidermis can be removed after the dermis is healed, and then further autologous skin transplantation, the US FDA has approved Integra was approved to be used in the treatment of burns and scalds in 1996; its main disadvantage is that due to the non-degradable silicone film on its surface, it is not easy to observe the early infection of the wound, which is not conducive to the crawling of wound edge epidermal cells and the sealing of the wound. The silicone membrane still needs a second operation