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Effective component of red paeonia and preparation and use thereof

An effective component, the technology of red peony root, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, which can solve the problems of side reactions, involvement of normal cells, etc.

Inactive Publication Date: 2009-01-21
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of red paeonia and preparation and use thereof
  • Effective component of red paeonia and preparation and use thereof
  • Effective component of red paeonia and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] The preparation of the effective component of embodiment 1 Radix Paeoniae Rubra

[0099] Take 250 g of Radix Paeoniae Rubra, crush it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extract to obtain 16.0g, the extract and silica gel are mixed as a sample, and the normal phase silica gel column is used for separation, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, Then, chloroform and methanol (10:1) were used as the mobile phase to obtain the eluent II, which was concentrated and dried to obtain 2.1 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is a Hanbang semi-preparative column (Lichrospher C18; 10.0mm×250mm, 5 μm), the mobile phase is water (A) and acetonitrile (B), and...

Embodiment 2

[0102] The analysis of the effective component of embodiment 2 Radix Paeoniae Rubra

[0103] The HPLC-ELSD joint analysis to the effective component of Radix Paeoniae Rubra of Example 1

[0104] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm × 150mm, 5 μm); using gradient elution, mobile phase A is 0.2% glacial acetic acid aqueous solution, mobile phase B is acetonitrile solution containing 0.2% glacial acetic acid; gradient elution The procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid in acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Aqueous solution, mobile phase B is 50% 0.2% glacial acetic acid acetonitrile solution; 30 minutes, mobile phase A is 5% 0.2% glacial acetic acid aqueous solution, mobile phase B is 95% 0.2% glacial acetic acid acetonitrile solution; 35 In minutes, the mobile phase A w...

Embodiment 3

[0107] Embodiment 3 Radix Paeoniae Rubra Active Component Preparation

[0108] Take 0.5 g of the effective component of Radix Paeoniae Rubra in Example 1 and mix evenly with 10.5 g of polyethylene glycol-6000, heat and melt, and move the material to drip irrigation after the material is made, and drop the medicine liquid into the liquid paraffin at 6-8 ° C, remove Oil, made 400 drop pills.

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PUM

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Abstract

The invention relates to an effective component of red peony root, a preparation method and application thereof; the preparation process of the effective component of red peony root in the invention includes the following steps: step 1, the mixture of ethyl acetate and ethanol is used as solvent for extracting red peony root; step 2, the extract is processed with chromatographic column chromatography to obtain eluate; step3, the eluate obtained by elution in the preparation of liquid phase chromatographic gradient is adopted; the mobile phase is water and acetonitrile, and the eluate of 28.6-32.2 min is collected to obtain the effective component.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Radix Paeoniae Rubra, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained fr...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P35/00C07D307/87C07H1/08A61K125/00
Inventor 贺庆刘雳史强程翼宇水文波葛志伟窦静霍阳
Owner TIANJIN TASLY PHARMA CO LTD
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