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Purine medicaments insensitive gene detecting kit

A detection kit and drug-sensitive technology, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of expensive equipment and instruments, complicated technology, and unsuitable economic promotion.

Active Publication Date: 2008-12-31
上海裕隆生物科技有限公司
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AI Technical Summary

Problems solved by technology

The equipment and instruments required by these methods are expensive and technically complex, and are not suitable for promotion as conventional methods from economic considerations.

Method used

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Embodiment Construction

[0037] The implementation of the object of the present invention will be described below in conjunction with the accompanying drawings and examples.

[0038] Implementation example:

[0039] 1. Sample Processing

[0040] Oral mucosal exfoliated cells were used as a sample source for genomic DNA extraction. For extraction, Biotech's Cell Genome Extraction Kit was used.

[0041] 2. Chip preparation, spotting the labeled positioning probes in the literature on ultra-flat glass slides, the probe sequences are as follows:

[0042] POS-1: ATTGATTGTGAATGAAATGAATTG

[0043] POS-2: GTAAATGATGATATTGGTATATTG

[0044] POS-3: ATTGTGAAGTATAAAGATGATTGA

[0045] POS-4: ATGAATTGAAAGTGATTGAAAAAG

[0046] POS-5: AAAGTGATGTATATGAGTAAATTG

[0047] POS-6: GTAGTAATGTTAATGAATTAGTAG

[0048] 3. Primer design:

[0049] (1) The following primer sequence set A was designed to amplify three gene fragments related to purine drug sensitivity on the TMPT gene, including mutation sites 238, 460 and 7...

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Abstract

The invention provides a purine drug sensitivity gene chip detection kit belonging to the gene chip technique field of clinical detection, which can be used to parallelly and economically detect genes related to purine drug sensitivity. The kit comprises an extraction solution, a hybridization buffer solution, a washing liquor, an amplifying solution, Taq enzyme and a gene chip, the amplifying solution contains a primer for amplifying genes of three mutant sites on mercaptopurine methyltransferase (TPMT), and the three mutant sites are respectively 238, 460 and 719. The purine drug is used for treating acute lymphoblastic leukemia, and the detection kit can be used to detect the three site mutation conditions of a TMPT gene sensitive to the purine drug.

Description

technical field [0001] The invention belongs to the technical field of gene chips for clinical detection, and in particular relates to a purine drug sensitive gene detection kit. Background technique [0002] The current research results show that 6-mercaptopurine and 6-mercaptopurine, the mercaptopurine metabolizing drugs for the treatment of acute lymphoblastic leukemia, are drugs without intrinsic biological activity, and must go through a series of metabolism in the body to exert their efficacy. The metabolizing enzyme of thiopurine methyltransferase (TPMT) in human body plays a key role in the metabolism and anti-leukemia effect of such drugs. The mutation sites contained in TPMT are related to the sensitivity of the human body to purine drugs. [0003] Currently, the methods used to detect single-point mutations of 5-fluorouracil mainly include: sequencing method, which is the most direct and accurate method; fluorescent quantitative PCR method, and single-base extens...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 穆海东汪宁梅杜喜玲
Owner 上海裕隆生物科技有限公司
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