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Detecting kit for cardiovascular and cerebrovascular diseases predisposing gene chip

A technology for cardiovascular and cerebrovascular diseases and susceptibility genes, which is applied in the field of cardiovascular and cerebrovascular disease susceptibility gene chip detection kits, and can solve the problems of complex technology, expensive equipment and instruments, and economic discomfort for promotion.

Inactive Publication Date: 2008-12-31
上海裕隆生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The equipment and instruments required by these methods are expensive and technically complex, and are not suitable for promotion as conventional methods from economic considerations.

Method used

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  • Detecting kit for cardiovascular and cerebrovascular diseases predisposing gene chip
  • Detecting kit for cardiovascular and cerebrovascular diseases predisposing gene chip

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Embodiment Construction

[0123] The implementation of the object of the present invention will be described below in conjunction with the accompanying drawings and examples.

[0124] Implementation example:

[0125] 1. Sample Processing

[0126] Oral mucosal exfoliated cells were used as a sample source for genomic DNA extraction. For extraction, Biotech's Cell Genome Extraction Kit was used.

[0127] 2. Chip preparation, spotting the labeled positioning probes in the literature on ultra-flat glass slides, the probe sequences are as follows:

[0128] POS-1: GATTTGTATTGATTGAGATTAAAG

[0129] POS-2: TGATTGTAGTATGTATTGATAAAG

[0130] POS-3: GATTGTAAGATTTGATAAAGTGTA

[0131] POS-4: GATTTGAAGATTATTGGTAATGTA

[0132] POS-5: GATTGATTATTGTGATTTGAATTG

[0133] POS-6: GATTTGATTGTAAAAAGATTGTTGA

[0134] POS-7: ATTGGTAAATTGGTAAATGAATTG

[0135] POS-8: GTAAGTAATGAATGTAAAAGGATT

[0136] POS-9: GTAAGATGTTGATATAGAAGATTA

[0137] POS-10: TGTAGATTTGTATGTATGTATGAT

[0138] POS-11: GATTAAAGTGATTGATGATTTGTA

...

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Abstract

The invention provides a cardiovascular and cerebrovascular diseases susceptibility gene chip detection kit belonging to the gene chip technique field of clinical detection, which can be used to parallelly and economically detect thirteen genes related to the susceptibility of cardiovascular and cerebrovascular diseases. The kit comprises an extraction solution, a hybridization buffer solution, a washing liquor, an amplifying solution, Taq enzyme and a gene chip, the amplifying solution contains a primer for amplifying the thirteen genes containing mutant sites, and the thirteen genes respectively are: an Enos gene, an ADD1 gene, an APOB-2515 gene, an AT1R gene, an APOB-3500 gene, an APOB-4181 gene, an eNOS-894 gene, an FgB-148 gene, an APOE gene, a GNB3 gene, an AGT gene, an FVII gene and an MTHFR gene. A plurality of genes are related to the susceptibility of the cardiovascular and cerebrovascular diseases, and the detection kit can be used for detecting the related thirteen genes parallelly.

Description

technical field [0001] The invention belongs to the technical field of gene chips for clinical detection, in particular to a detection kit for cardiovascular and cerebrovascular disease susceptibility gene chips. Background technique [0002] There are many susceptibility genes related to cardiovascular and cerebrovascular diseases. By consulting the literature, 13 genes with strong correlation were selected, namely Enos gene, ADD1 gene, APOB-2515 gene, AT1R gene, APOB-3500 gene, APOB -4181 gene, eNOS-894 gene, FgB-148 gene, APOE gene, GNB3 gene, AGT gene, FVII gene, MTHFR gene. It is used to carry out relevant research on the susceptibility genes of cardiovascular and cerebrovascular diseases. [0003] At present, the parallelism used for the study of cardiovascular and cerebrovascular disease susceptibility genes does not reach more than 13 genes. The susceptibility genes of this kit are comprehensively summarized. In addition, the detection methods for point mutations i...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 穆海东汪宁梅杜喜玲
Owner 上海裕隆生物科技有限公司
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