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Space-sharing hybridization reaction device for biological chip

A biochip and hybridization reaction technology, applied in biochemical cleaning devices, enzymology/microbiology devices, bioreactor/fermenter combinations, etc., can solve the problem of simplification, automation and high throughput of biochips, scanning images The background is too strong, the operation is complicated, etc., to achieve the effect of improving the automation level and high-throughput performance, and the operation of hybridization reaction and signal scanning is simple.

Active Publication Date: 2008-12-03
DAAN GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation of removing the fence is complicated and must be operated manually, thus affecting the automation of the instrument
In addition, the background of the scanned image is too strong or dirty due to the residue of sticky colloid
On the other hand, due to the existence of relatively random fences, only single-head pipettes can be used to add samples during operation, resulting in slow sample addition and error-prone
These factors undoubtedly limit the simplification, automation and high-throughput of biochip applications.

Method used

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  • Space-sharing hybridization reaction device for biological chip

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Effect test

Embodiment 1

[0020] Embodiment 1, biochip partition hybridization reaction device

[0021] As shown in Fig. 1-2, the present invention is a kind of partition hybridization reaction enclosure specially for the biochip with glass slide as the carrier, and the fence is designed with three hollowed-out scanner card position support points (1), each hollowed out The shape of the zone is circular, oval, square, triangular, with an area of ​​up to 1mm 2 or 1mm 2 above. During operation, the card contact point of the scanner should be aligned with the hollow area (1). When scanning the biochip with the fence of the hollow support point, it can be scanned directly without removing the film, and can obtain stable focus, while avoiding the trouble of manually removing the film and leaving residual marks after removing the film The problem.

[0022] The fence also has 8 rows of hollowed-out hybridization reaction areas (2), the spacing of the 8-row reaction areas is the same as that of standard 8-...

Embodiment 2

[0024] Embodiment 2, the use method of biochip partition hybridization reaction device

[0025] The use process of this reaction device is as follows:

[0026] Paste the partition fence of the present invention on the glass slide after the surface chemical treatment, and press it tightly without leaving any gaps. Then, use a spotting robot to spot microarray probes in each hybridization area, and leave at room temperature for 12 hours to chemically bind the probes to the glass slide. Use the washing solution to wash away the unbound probes and then blow dry for later use. Prepare hybridization sample templates in groups of 8, and place the templates in 96-well plates or 384-well plates or 8-strip tubes. Using an 8-channel pipette, add templates in groups of 8 to the hybridization area of ​​the chip, and then add hybridization solution in batches in groups of 8. Place the chip on the automatic hybridization instrument, set the hybridization temperature, hybridization time, w...

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Abstract

The invention relates to a biochip hybridization sectioning device which is a fence special for the biochip, particularly a sectioning device which is arranged on a fence so as to facilitate scanning and high-flux sample-application detecting. The device is suitable for the hybridization reaction sectioning arrangement of all the biochips that adopt a slide as the carrier; and the biochip hybridization sectioning device has the remarkable advantages over the conventional design and device in scanning simplicity and rapidness, the flux of the hybridization reaction operation and the automation.

Description

Technical field [0001] The invention relates to a biological chip hybridization partitioning device, in particular to a partitioning device which can control the hybridization reaction area of ​​the biological chip and is convenient for scanning. technical background [0002] Biochip refers to a microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as glass slides, silicon wafers, and nylon membranes. The chip is hybridized with the labeled sample, and the analysis of the biological sample can be realized by detecting the hybridization signal. Biochip technology integrates many discontinuous analysis processes (such as sample preparation, chemical reaction and analytical detection) into the chip by adopting microelectronics, micromechanics, special chemical treatment, biological manipulation and other processes and processes to make it continuous and integrated. miniaturization and miniaturi...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 胡守旺张帆胥顺程钢何蕴韶
Owner DAAN GENE CO LTD
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