Method for preparing nano-multiple phase calcium phosphate/fibroin protein composite bracket

A multi-phase calcium phosphate and nano-hydroxyapatite technology, applied in medical science, prosthesis, etc., can solve the problems of unfavorable bone cell adhesion, differentiation, proliferation, growth, poor stomatal connectivity, small stomatal size, etc.

Inactive Publication Date: 2013-06-05
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing methods for preparing scaffolds, either the introduction of toxic organic solvents damages the biological activity of the material, or the internal structure of the material has small pore size, low porosity, and poor pore connectivity, which is not conducive to the adhesion, differentiation, and formation of bone cells. Proliferation and growth; the strength of the prepared material is too low to meet the needs of bearing bone defect repair

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0007] Concrete embodiment one: the operation steps of this embodiment are: a. use calcium chloride and diammonium hydrogen phosphate as raw materials for generating calcium-deficient hydroxyapatite, the concentration of the reaction solution is 0.2M, and the particle size is 90-300 μm Silk fibroin powder is added as a template in calcium chloride solution to regulate the morphology of HA. According to the mass ratio, the mass ratio of HA to SF is (7-3): (3-7), reacted at different temperatures, ammonia water and hydrochloric acid adjusted The pH value of the reaction solution is 7-10, react for 2-3 hours, age for 20 hours, and then centrifuge and dry to obtain HA / SF composite powder; b. Compress the HA / SF composite powder and sinter it in an air furnace at atmospheric pressure. The sintering temperature is 900-1100°C, and the holding time is 2-3 hours to obtain a porous nano-composite calcium phosphate ceramic matrix; c. impregnate the porous nano-composite calcium phosphate c...

Embodiment approach 2

[0008] Concrete embodiment two: the operation steps of this embodiment are: a. use calcium chloride and diammonium hydrogen phosphate as raw materials for generating hydroxyapatite, add distilled water to prepare a reaction solution with a concentration of 0.2M and a particle size of 90 to 300 μm The silk fibroin powder was added as a template in the calcium chloride solution to regulate the morphology of HA. According to the mass ratio, the mass ratio of HA and SF was 7:3, reacted at room temperature, and the pH value of the reaction solution was adjusted by ammonia water and hydrochloric acid to 10. React for 2 hours, age for 20 hours, and then centrifuge and dry to obtain HA / SF composite powder; b. Compress 2 g of HA / SF composite powder under 8 MPa pressure into The green body is sintered in an air furnace at normal pressure, the sintering temperature is 900 ° C, and the holding time is 3 hours, and a porous nano-composite calcium phosphate ceramic matrix is ​​obtained, the...

Embodiment approach 3

[0009] Concrete embodiment three: the operation steps of this embodiment are: a. use calcium chloride and diammonium hydrogen phosphate as raw materials for generating hydroxyapatite, add distilled water to prepare a reaction solution with a concentration of 0.2M and a particle size of 90 to 300 μm The silk fibroin powder was added as a template to the calcium chloride solution to regulate the morphology of HA. According to the mass ratio, the mass ratio of HA and SF was 5:5, and reacted at 40 ° C. The pH value of the reaction solution was adjusted by ammonia water and hydrochloric acid. React for 3 hours, age for 20 hours and then centrifuge and dry to obtain HA / SF composite powder; b. Compress 2 g of HA / SF composite powder under a pressure of 8 MPa to form The green body is sintered in an air furnace at atmospheric pressure, the sintering temperature is 1000°C, and the holding time is 3 hours, to obtain a porous multiphase calcium phosphate ceramic matrix with a porosity of ...

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Abstract

A preparation method of nanometer multiphase calcium phosphate / fibroin protein composite bracket relates to a preparation method of tissue engineering cytoskeleton. The existing preparation technology has the defects that organic solvent goes against the introduction of active material, sealed stomata goes against the growth of osseous tissue, low mechanical strength is unsuitable for bearing bone reparative regeneration and tissue distribution is uneven. The method provided by the present invention includes: a. calcium-deficient nanometer hydroxyapatite powder is synthesized by the effect offibroin organic template; b. pore-foaming agent sintering technique is adopted to crack the calcium-deficient nanometer hydroxyapatite powder after press molding for further preparation of nano-porous multiphase calcium phosphate ceramic matrix, the sintering temperature is 900 to 1,100 DEG C, the time is 2 to 3 hours and the pore-foaming agent is fibroin powder with the gain diameter of 90 to 300Mum; c. the matrix is dipped in 1 to 5 percent fibroin solution, frozen and dried to obtain the nanometer multiphase calcium phosphate / fibroin protein composite bracket material. The low-temperature sintering of the method provided by the present invention maintains the biological activity of the ceramics and overcomes the defects of the existing technology.

Description

Technical field: [0001] The present invention relates to a preparation method of tissue engineering porous composite cell scaffold, in particular to a kind of calcium-deficient nano-hydroxyapatite (HA) pyrolysis to prepare nano-composite calcium phosphate (BCP) ceramic matrix with uniform distribution of macroscopic porous tissue, and then impregnated A method for preparing a nano-BCP / SF composite scaffold with a high specific surface area by freeze-drying a silk fibroin (SF) solution. Background technique: [0002] Porous HA can induce the growth of surrounding bone tissue and form a firm chemical bond with the bone. It has excellent osteoconductivity, can be better integrated into the host bone, has a strong affinity for bone morphogenetic proteins, and is non-toxic Side effects and no carcinogenic effect, so it is widely used as a physiological scaffold for hard tissue repair materials, but the extremely low degradation rate cannot make it used as a degradable material, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/42
Inventor 李慕勤王静孟祥才曲立杰
Owner JIAMUSI UNIVERSITY
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