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Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation

A technology for the metabolic characteristics of Micrococcus luteus, applied in the aerobic biodegradation of picolinic acid, in the field of Micrococcus luteus, can solve the problems of non-degradability, achieve the effect of enhancing processing capacity and broad application potential

Inactive Publication Date: 2008-09-10
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to domesticate and screen out the efficient degradation of nitrobenzene through scientific methods in view of the characteristics of high salinity in the actual nitrobenzene industrial wastewater and the problem of the non-degradable by-product picolinic acid in the partial reduction process of nitrobenzene Bacteria to treat nitrobenzene wastewater with high salinity and solve the bottleneck in the degradation process of nitrobenzene

Method used

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  • Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation
  • Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation
  • Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation

Examples

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Embodiment 1

[0030] Micrococcus luteus provided by the invention, its screening steps are as follows:

[0031] Samples of aerobic activated sludge were collected from the aeration tank of Dalian Dyestuff Factory, and used as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate to 50ml of activated sludge as a deflocculant, vibrate on a shaker for 5-10min to break up the activated sludge flocs, take 5ml of activated sludge, add 95ml of inorganic salt medium to shake In the bottle, add nitrobenzene as the only carbon, nitrogen, and energy source, wherein the concentration of nitrobenzene is 200 mg / L, and carry out shaker cultivation at a temperature of 30° C. and a rotation speed of 180 r / min. Inorganic salt medium composition: Na 2 HPO 4 12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 2H 2 O, 10mg / L; FeCl 3 , 2mg / L; MgSO 4 ·7H 2 O, 20mg / L. When the bacterial liquid becomes turbid and nitrobenzene cannot be detected, the next transfer is carried out; after...

Embodiment 2

[0048] Among the present invention, the application of Micrococcus luteus p-nitrobenzene aerobic degradation research, its steps are as follows:

[0049] [1] Add 100ml of inorganic salt medium to a 250ml Erlenmeyer flask, then add 200mg / L of nitrobenzene as the only carbon, nitrogen, and energy source, and sterilize at 121°C;

[0050] [2] Add the liquid culture of Micrococcus luteus cells prepared in Example 1 into the medium of [1] above, 30°C, 180r / min, aerobic culture, figure 1 The growth of Micrococcus luteus and the degradation of nitrobenzene.

Embodiment 3

[0052] In the present invention, the application of Micrococcus luteus to the research of picolinic acid aerobic degradation, its steps are as follows:

[0053] [1] Add 100ml of inorganic salt medium to a 250ml Erlenmeyer flask, add 2000mg / L picolinic acid as the only carbon, nitrogen, and energy source, and sterilize at 121°C;

[0054] [2] Add the liquid culture of Micrococcus luteus cells prepared in Example 1 into the medium of [1] above, 30°C, 180r / min, aerobic culture, figure 2 Growth of Micrococcus luteus and degradation of picolinic acid.

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Abstract

The invention relates to a gamboges micrococcus provided with novel metabolism characteristics and application of the gamboges micrococcus in biodegradation, belonging to the bioengineering technical field. The invention produces a gamboges micrococcus provided with the novel metabolism characteristics by means of separation sieve. The invention has the advantages that: the gamboges micrococcus degrades nitrobenzene by means of partial reduction and mineralizes by-products - picolinic acids during the degradation process, thereby complete degradation of the nitrobenzene is realized and the problem during the process of partial reduction degradation of the nitrobenzene is solved; growth of the gamboges micrococcus under the condition of high salinity is not affected, thereby the nitrobenzene is completely degraded; the gamboges micrococcus can take the picolinic acids as a single carbon source, a single nitrogen source and a single energy source for growth, and the picolinic acids are finally mineralized into innoxious carbon dioxides and water; the gamboges micrococcus can be used as biological enhancing preparations and applied in biological treatment of nitrobenzene waste water and picolinic acid waste water with high salinity.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a strain of Micrococcus luteus with new metabolic characteristics and its application in the aerobic biodegradation of nitrobenzene and picolinic acid. Background technique [0002] Nitrobenzene is widely used in the production of medicine, dyes, plastics, explosives, etc. The wide application of nitrobenzene leads to the pollution of the environment by nitrobenzene. Nitrobenzene wastewater can be treated by aerobic biological method. At present, microbial resources for aerobic degradation of nitrobenzene include bacteria and white rot fungi, and bacteria include Pseudomonas, Comamonas, Bacillus, Corynebacterium, Staphylococcus, Streptococcus, etc. [0003] The actual nitrobenzene industrial wastewater has the characteristics of high salinity and complex composition. High salinity wastewater is wastewater with a total dissolved solids (sodium chloride) of at least 3.5% (W / V...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/265
Inventor 周集体郑春莉王竞吕红曲嫒嫒金若菲项学敏张劲松王静
Owner DALIAN UNIV OF TECH
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