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Extraction of jellyfish collagen and method for producing collagen peptide

A collagen and jellyfish technology, which is applied in the field of jellyfish collagen extraction and collagen peptide preparation, can solve the problems of unsuitable industrial production, high requirements, long production cycle and the like, and achieves short production cycle, simple production process and low cost. Effect

Inactive Publication Date: 2008-08-20
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rate and purity of collagen extracted by this method are high, but the operation is cumbersome, the production cycle is long, the requirements are high, the cost is high, and it is not suitable for industrial production
[0006] So far, there have been no reports on acid-base swelling, hot water extraction of jellyfish collagen and enzyme engineering technology to obtain active peptides with different molecular weights

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0009] Specific implementation method 1: Wash the fresh jellyfish with water, remove the adhered sediment impurities and mucus formed by the autolysis of the jellyfish, and remove the internal organs. Wash with tap water, mechanically cut into pieces, add a certain amount of pretreated jellyfish to 1 times the volume of 1% NaOH, soak for 12 hours, wash with water until neutral, add 1 times the volume of 1% H 2 SO 4 Soak for 6 hours, wash with water and homogenize; adjust the pH of the homogenate to 5.0 and heat to 70°C, and continuously stir and extract at this temperature for 8 hours, then centrifuge and filter; add 2 times the volume of water to the residue, extract at 80°C for 3 hours, Centrifuge; combine the two extracts. The extract is desalted, concentrated and dried to obtain a collagen product.

specific Embodiment approach 2

[0010] Specific embodiment 2: The frozen jellyfish sample is thawed, washed with tap water, mechanically chopped into pieces. Add a certain amount of pretreated jellyfish to 2 times the volume of 1% NaOH, soak for 6 hours, wash with water until neutral, add 2 times the volume of 1% H 2 SO 4 Soak for 3 hours, wash with water, and homogenate; heat the homogenate to 70°C under acidic conditions, and continuously stir and extract at this temperature for 8 hours, then centrifugally filter; add 2 times the volume of water to the residue, and extract at 80°C for 3 hours, Centrifuge; combine the two extracts. Prepare the extract to 0.5molL -1 Acetic acid solution, add 2% pepsin, enzymolyze at 37°C for 4h, inactivate enzyme at 85-100°C for 5-10min, cool to room temperature, centrifuge, use ultrafiltration technology to collect filtrates of different molecular weight segments, desalinate, concentrate and dry Obtain active peptide products.

specific Embodiment approach 3

[0011] Specific embodiment 3: Wash the salted jellyfish sample with tap water, mechanically cut it into pieces, add a certain amount of pretreated jellyfish to 6 times the volume of 0.5% NaOH and soak for 0.5h, wash with water until neutral, add 6 times the volume of 0.2% H 2 SO 4 Soak for 1 hour, wash with water, and homogenate; heat the homogenate to 70°C under acidic conditions, and continuously stir and extract at this temperature for 8 hours, then centrifuge and filter; add 2 times the volume of water to the residue, and extract at 80°C for 3 hours, Centrifuge; combine the two extracts. Adjust the pH of the extract to 7.0, add 3% trypsin at 45°C, stir at a constant temperature, and enzymatically hydrolyze for 3 hours; adjust the pH of the reaction solution to 8.5 with high-concentration sodium hydroxide, add 4% alkaline protease, and heat at 50°C Stir at a constant temperature for 4 hours, inactivate the enzyme at 85-100°C for 5-10 minutes, cool to room temperature, cent...

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Abstract

The invention relates to a preparation method of jellyfish collagen protein and a method for utilizing the enzyme engineering technology to obtain active peptides with different molecular weight segments. The method takes fresh jellyfish, unfrozen jellyfish or salted jellyfish as raw materials, the acid and base are adopted to carry out the swelling treatment of the raw materials, the jellyfish collagen protein is extracted by hot water, and the collagen is obtained by desalination, concentration and drying. The extracted collagen protein is subject to directional enzymolysis, ultrafiltration separation, desalination, concentration and drying, so the ladder collagen peptides with the different molecular weights can be obtained. The collagen protein and the peptides which are prepared by the invention has high extraction rate, short production cycle, simple production process, controllable product specifications and low cost, which is applicable to the industrial production. The products prepared by the invention are white or light yellow, good in quality and very easy to be dissolved in water, so the products can be used in the fields of medical health care, cosmetics and food, etc. and provide a new idea for the storage and the high-valve utilization of jellyfish.

Description

Technical field: [0001] The invention relates to a method for preparing jellyfish collagen by acid-base treatment and hot water extraction and a method for obtaining active peptides of different molecular weight segments by using enzyme engineering technology. The invention makes full use of jellyfish resources, and proposes new ideas for storage and high-value utilization of jellyfish. The preparation method of the invention has high extraction rate of collagen and polypeptide, short production cycle, simple production process and low cost. The product is white or light yellow, good quality, easily soluble in water, and can be used in fields such as medical care, cosmetics and food. Background technique: [0002] Jellyfish not only has many types and large numbers, but also has a wide distribution. It is an important part of marine zooplankton communities and occupies an important position in marine biological ecosystems. Jellyfish, as a large organism with the same origi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C12P21/06
Inventor 李八方庄永亮赵雪
Owner OCEAN UNIV OF CHINA
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