Biological fertilizer and manufacture method thereof
A technology of bio-fertilizer and enzyme bacteria, applied in the field of bio-fertilizer, can solve the problems of poor compatibility, insignificant comprehensive treatment effect, poor efficiency of urban sludge, etc., and achieve low cost, good complexation adsorption performance, and strong adsorption force Effect
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[0040] 1. Isolation of Bacillus megaterium M1
[0041] Evenly mix 5-10g of commercially available enzyme bacteria with 5-10g of nutrient-based cornstarch, 5-10g of brown sugar and 10-20g of wheat bran, pH6.0-7.0, humidity control at 30-35%, and then add hydroxylamine or ethyl alcohol The imine is 0.005-0.05g, the temperature is 25-35°C, and the temperature difference is 8-10°C. After 2-3 days of cultivation, the Bacillus megaterium M1 is screened out with an inoculation loop. The nutrient base may also include at least one of 3-5 g of fish meal, 3-5 g of vegetable cake and 35-50 g of polished rice bran.
[0042] 2. Cultivation of Bacillus megaterium M1
[0043] Bacillus megaterium M1 grows on the following media:
[0044] Sucrose 50g (NH 4 ) 2 SO 4 6g NgSO 4 2.5g (NH 4 ) 3 SO 4 2g
[0045] K 2 SO 4 2.5g CaSO 4 2.5g agar 1520g distilled water 1000ml pH6.0
[0046] 3. the preparation of biological fertilizer of the present invention
[0047] The novel enzyme ba...
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