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Chinese herbal medicine residual organophosphorus pesticide detection method

An organophosphorus pesticide and detection method technology, applied in the detection field, can solve the problems of chromatographic column pollution, difficult to carry out detection items, poor repeatability, etc.

Active Publication Date: 2008-08-13
JING BRAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the first disadvantage is that an expensive multifunctional vacuum sample processor needs to be configured, which is unaffordable for ordinary enterprises, which limits the popularization and application of this detection method, and makes it difficult to carry out the detection items of organophosphorus pesticides in Chinese medicinal materials. Safety medication leaves hidden dangers
The second is that the purification effect is poor. The specific performance is that the activated carbon column has an unsatisfactory effect on removing the pigment in the sample, resulting in a relatively large degree of damage to the inlet system, liner, sample needle, and chromatographic column when entering the chromatographic analysis. Contamination increases instrument operating costs and poor repeatability

Method used

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  • Chinese herbal medicine residual organophosphorus pesticide detection method
  • Chinese herbal medicine residual organophosphorus pesticide detection method
  • Chinese herbal medicine residual organophosphorus pesticide detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Detection of Residual Organophosphorus Pesticides in Radix Astragali Medicinal Materials

[0037] One, the preparation of reference substance solution

[0038] Accurately absorb 40ul of acephate and omethoate standard stock solution with a concentration of 1000ug / ml provided by the National Environmental Monitoring Station of the Ministry of Agriculture, and dichlorvos, methamidophos, demeton-methyl, phorate, and diazinon , monocrotophos, dimethoate, pirimiphos-methyl, chlorpyrifos, methyl parathion, fenthion, parathion, malathion, parathion, ethion (acetone) standard stock solution each 20ul in In a 10ml volumetric flask, set the volume to the mark with acetone, and mix thoroughly on a vortex shaker to obtain acephate and omethoate with a concentration of 4ug / ml and other 15 standard stock solutions with a concentration of 2ug / ml. The mixed solution of organophosphorus reference substance was stored in the refrigerator for later use.

[0039] 2. Main experi...

Embodiment 2

[0064] Example 2 Detection of Residual Organophosphorus Pesticides in Angelica Medicinal Materials

[0065] One, the preparation of reference substance solution is the same as embodiment 1

[0066] Two, experimental apparatus, chromatographic condition and reagent are the same as embodiment 1

[0067] Three, the detection of reference substance solution is the same as embodiment 1

[0068] Fourth, the preparation of the test article

[0069] Accurately weigh 5g of angelica (origin, Gansu, batch number 20060510) medicinal powder, pass through a No. 2 sieve, add 5g of anhydrous sodium sulfate, 0.2g of activated carbon, add 50ml of ethyl acetate, shake for 30min, let stand overnight, and ultrasonicate for 5min in an ice bath , place it, take the upper layer and filter, add 30ml of ethyl acetate to the residue, sonicate in an ice bath for 5min, let it stand, filter into the last filtrate, wash the residue and filter paper with 10ml of ethyl acetate, and combine with the above fi...

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Abstract

The present invention provides a method for examining residual organophosphorus pesticides in Chinese traditional medicine, including steps of: (1) preparing a certain concentration standard solution containing 17 organochlorine pesticides; (2) examining standard solution under the propriety chromatogram conditions to obtain standard chromatogram graph; (3) preparing specimen: dipping specimen powder in ethyl acetate, ultrasonic extracting twice and purified and decoloring by PSA columella, decompressing and concentrating; (4) examining specimen under the same chromatogram conditions of reference substance to obtain chromatogram graph of specimen; (5) comparing the chromatogram graph between specimen and reference substance by preservation time contrast method to determine corresponding characteristic peak to calculate content by external standard method. The present invention is easy to application and dissemination, which has good purification effects to solve the problem of pollution of coloring matter to charomatograph. The present invention has high sensitivity, of which the recovery is 64%-110%; and good reproducibility and the warp is less than 15%.

Description

(1) Technical field: [0001] The invention relates to a detection method, in particular to a detection method for residual organophosphorus pesticides in Chinese medicinal materials. (two) background technology: [0002] Organophosphorus pesticides are a class of phosphorus-containing organic compounds. There are many types. At least 60 kinds are produced and used in large quantities. According to their toxicity, they can be classified as highly toxic (such as parathion, commonly known as 1605, dichlorvos, phorate, etc ), medium toxicity and low toxicity. Organophosphorus pesticides have the advantages of small dosage, high insecticidal efficiency, strong selective effect, little harm to crops, economical use, and because of their unstable properties, they are easy to decompose in nature, have a very short residual time in crops, and are in vivo It is easily hydrolyzed by enzymes and does not accumulate in the body, so it is widely used. Due to the relatively long planting ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08G01N1/28
Inventor 孙细珍
Owner JING BRAND
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