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Cement-base material microorganism tectorial membrane protection agent, preparing method and application thereof

A cement-based material and protective agent technology, applied in the biological field, can solve the problems of cement-based material surface performance degradation, easy loosening and peeling of the surface, acceleration of concrete durability, etc., to achieve excellent performance, significant protective effect of coating, good The effect of weather resistance

Active Publication Date: 2008-08-06
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Existing technology: Due to the existence of various erosive media in the service environment of cement-based materials, during the material preparation process and service period, due to the joint action of load and external factors, the surface of cement-based materials is prone to problems such as looseness and peeling, resulting in The surface properties of cement-based materials gradually decrease
If these problems cannot be repaired in time, external water and aggressive media will continue to infiltrate from the surface of the material, which will eventually lead to accelerated deterioration of the durability of concrete.

Method used

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  • Cement-base material microorganism tectorial membrane protection agent, preparing method and application thereof
  • Cement-base material microorganism tectorial membrane protection agent, preparing method and application thereof
  • Cement-base material microorganism tectorial membrane protection agent, preparing method and application thereof

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Effect test

Embodiment 1

[0027] Inoculate the strain Bacillus pasteurii into the culture solution containing the urea-containing substrate. The composition is shown in Table 1. Shake culture at 25-37°C, take it out after 16-24 hours, and centrifuge at 5000-8000rpm 5-8 minutes, remove the supernatant, collect the cells of the strain with the culture medium, measure the OD value of the transmittance of the concentrated bacterial liquid with a UV-visible light spectrophotometer, and divide the collected strain cells according to the linear calibration curve between the OD value and the concentration of the bacterial liquid Concentration control is 4×10 10 cell / mL. Then prepare urea and Ca(NO 3 ) 2 Mixed solution, the concentration is 5mol / L, add 2g agar powder per liter of mixed solution, mix and heat to 90°C, after the agar is completely dissolved, put it at room temperature and cool to 50°C, then mix with the concentrated bacterial solution collected by centrifugation according to 1 Stir and mix at ...

Embodiment 2

[0029] Inoculate the strain Bacillus pasteurii into the culture solution containing the urea-containing substrate. The composition is shown in Table 1. Shake culture at 25-37°C, take it out after 16-24 hours, and centrifuge at 5000-8000rpm 5-8 minutes, remove the supernatant, collect the cells of the strain with the culture medium, measure the OD value of the transmittance of the concentrated bacterial liquid with a UV-visible light spectrophotometer, and divide the collected strain cells according to the linear calibration curve between the OD value and the concentration of the bacterial liquid Concentration control is 4×10 9 cell / mL. Then prepare urea and Ca(NO 3 ) 2 Mixed solution, the concentration is 5mol / L, add 2g agar powder per liter of mixed solution, mix and heat to 90°C, after the agar is completely dissolved, put it at room temperature and cool to 50°C, then mix with the concentrated bacterial solution collected by centrifugation according to 1 Stir and mix at a...

Embodiment 3

[0031] A protective agent for microbial coating of cement-based materials. The protective agent is composed of microbial bacterial liquid capable of synthesizing minerals and a coagulant, wherein the volume ratio of the microbial bacterial liquid and the coagulant is 1:1. The microbial bacterial liquid is Bacillus pasteurii, and the bacterial liquid concentration is 4×10 9 cell / mL. The coagulant is urea, Ca(NO 3 ) 2 A mixed solution with agar, in which the concentration of urea is 3mol / L, Ca(NO 3 ) 2 The concentration is 3mol / L, and each liter of mixed solution contains 1.5g of agar powder.

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Abstract

A cement-based material microbe covering film repellent is characterized in that the cement-based material microbe covering film repellent consists of microbe bacteria agent and coagulator which can be used for synthesizing minerals; wherein, the cubage proportion of microbe bacteria agent to coagulator ranges from 1 to 2 : 1. The invention makes full use of natural microbe resources to mineralize and precipitate a layer of compact calcium carbonate on the surface of samples through the mineralization effect of creatures. The cement-based material microbe covering film is characterized by not only rich resources but also clean environment, low cost, and simple technique. Being an inorganic material, the calcium carbonate has the good weatherability and good compatibility with base materials, is able to automatically balance internal various stresses (temperature stress and crystallization stress) of base materials, and has a smaller impact on base materials after the protective material loses effect.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a protective agent for microbial coating of cement-based materials, a preparation method and application thereof. 2. Background technology [0002] Existing technology: Due to the existence of various erosive media in the service environment of cement-based materials, during the material preparation process and service period, due to the joint action of load and external factors, the surface of cement-based materials is prone to problems such as looseness and peeling, resulting in The surface properties of cement-based materials gradually decrease. If these problems cannot be repaired in time, external water and aggressive media will continue to infiltrate from the surface of the material, which will eventually lead to accelerated deterioration of the durability of concrete. Concrete surface and internal defect repair has become an important independent industry. Many...

Claims

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Application Information

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IPC IPC(8): C04B41/45C04B41/61
CPCC04B41/501C04B41/009C04B41/5009
Inventor 钱春香王瑞兴王剑云成亮
Owner SOUTHEAST UNIV
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