Method for extracting and separating prunol from Mesona Procumbens Hemsl

A technology of ursolic acid and fairy grass, which is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, plant/algae/fungus/moss components, etc., can solve the problems of lack of systematic research on ursolic acid extraction, waste of resources, etc. , to achieve the effect of low cost, high extraction efficiency and simple process

Inactive Publication Date: 2011-05-11
刘富来
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because it is insoluble in water, ursolic acid, which has the effect of beauty, whitening and health care, exists in the jelly jelly dregs after extracting jelly jelly and is discarded as waste, resulting in a huge waste of resources
Current research on the lack of system for extracting ursolic acid from Grass jelly

Method used

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Embodiment Construction

[0023] The present invention will be described in further detail below.

[0024] The concrete steps of the method for extracting and isolating ursolic acid from the whole herb of celestiaceae described in the present invention are:

[0025] 1) extract

[0026] ①Take the whole herb powder of jelly jelly, in a water bath at 85°C, use 90% ethanol solution as the extraction solution, the liquid-solid ratio is 18:1, extract twice, each time for 2.5h, filter, and extract Put it in a rotary evaporator, recover ethanol, add 6-8 times the amount of 50% chloroform aqueous solution to extract the residue, two hours each for the first two times, and one hour each for the last two times, combine the chloroform layer extracts, and leave overnight;

[0027] ② After filtering, concentrate the filtrate, recover the solvent to a small volume, place the precipitate to obtain the crude extract of ursolic acid;

[0028] ③The crude extract of ursolic acid was washed 3 times with water, filtered, ...

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PUM

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Abstract

The invention discloses a method for extracting and separating ursolic acid from a Mosla dianthera Maxim, which can be shown as follows: (1) extracting the Mosla dianthera Maxim crude powder twice in the water of 85 DEG C with 90 percent (volume percent) of ethanol and solution as the an extract and a liquid-solid ratio of 18:1; filtrating at the point of 2.5h with the extract placed in a rotary evaporator; recycling the ethanol and extracting the residues for three times after adding 6 to 8 times of volume dose of 50 percent chloroform aqueous solution with the former twice for two hours respectively and the latter twice for one hour respectively; combining the extract on the chloroform layers and placing for a whole night; (2) condensing the filtrate after filtrating, placing the deposition to get the ursolic acid crude extracts after recovering the solvent into small volumes; (3) washing the ursolic acid crude extracts with water for three times, filtrating and throwing away the filtrate, using an acetone to scour off the impurity from the deposition with multiple-low-dose; (4) thermally filtrating after dissolving with an anhydrous ethanol and placing for crystallization; (5) recrystallization in the same way; (6) finally, recrystallizing with the methanol-ethanol mixed solvent and placing to derive white needle shaped crystal. The method has the advantages of simplicity, low-cost and high yield, which is suitable for industrial production.

Description

technical field [0001] The invention discloses a method for extracting and isolating ursolic acid from plants, in particular to a method for extracting and isolating ursolic acid from whole grass jelly. Background technique [0002] Grass jelly, also known as jelly grass, fairy grass, fairy jelly, fire grass, etc., is an annual herb of the Lamiaceae genus Xiancao. Dried grass jelly contains about 70% carbohydrates, a small amount of protein, fat, pigment, etc., and more mineral elements. Among the functional ingredients contained in Grass jelly, quercetin can inhibit the growth of cancer cells and lower blood pressure; polysaccharides can enhance and improve the immune function of the body; The development potential lipid-lowering and liver-protecting drugs are also the main whitening ingredients in beauty products; essences have the effects of calming, cooling, detoxifying and diuretic; trace elements can inhibit the formation of free radicals, anti-aging, and anti-cancer ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61K36/53
Inventor 刘富来
Owner 刘富来
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