Infrared laser desorption/vacuume ultraviolet single photon ionization mass spectrometry analytical equipment

Abstract

The invention relates to a novel IR laser desorption / vacuum UV single-photon ionization mass spectrum analyzing device for analysis and detection of a biological sample, which solves the problems of the prior art that uses matrix and is unlikely to analyze complex mixture with low polarity and low molecular weight. The device is structurally characterized in that a photoionization chamber is arranged on one side of a time-of-flight mass spectrometer, an ion injection hole is provided on one sidewall of the photoionization chamber, an ion injector and a sample target are arranged in the chamber, a laser is disposed outside the photoionization chamber on the sample target side, and vacuum UV light source is arranged on the other side. By combining IR laser desorption technology and vacuum UV ionization mass spectrum technology, the invention can achieve high-efficiency and rapid analysis without selecting different matrixes according to the sample and without complex pre-treatment and separation of the sample, thus preventing the matrix interference on mass spectral signals. The invention is specifically suitable for analysis of thermal-unstable and nonvolatile organic compounds and biological molecules.

Description

technical field [0001] The invention relates to an analysis and detection device for organic substances, medicines, complex natural products and biological samples, in particular to a photoionization mass spectrometry analysis device. Background technique [0002] Mass spectrometry is an important method for identifying molecular structures. According to the structure and properties of the analyte, there are various kinds of mass spectrometry sampling, separation systems, ionization techniques and mass analyzers. Matrix-assisted laser desorption / ionization mass spectrometry (MALDI-TOFMS) is a technology that ingeniously combines matrix-assisted laser desorption / ionization and time-of-flight mass spectrometry (TOF). an important direction. [0003] Matrix-assisted laser desorption / ionization mass spectrometry (MALDI-TOFMS) first requires that the sample solution and a specific matrix solution are fully mixed, and after the solvent evaporates, it becomes a semi-crystalline o...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings that the existing matrix-assisted laser desorption / ionization mass spectrometry device must use a matrix, which is prone to fragment ion interference and cannot realize the analysis of some low polarity, small molecular weight and complex organic mixtures, the present invention provides an infrared desorption laser A new infrared laser desorption / vacuum ultraviolet single photon ionization mass spectrometry device that is separated from vacuum ultraviolet ionization light in time and space without using a matrix

Images