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Antineoplastic activity protein in syngnathus acus linnaeus

A technology of anti-tumor activity and active protein, applied in the field of anti-tumor active protein in Jianhailong, to achieve the effect of strong inhibition, improvement of physical symptoms and good anti-tumor activity

Inactive Publication Date: 2008-07-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the whole body of Jianhailong is still used as medicine for the treatment of bruises and impotence and nocturnal emission. There is no report about the anti-tumor active protein of Jianhailong.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The anti-tumor active protein of Jianhailong provided in this example is white powder in appearance.

[0019] SDS-PAGE was performed on the protein, the concentration of the separating gel was 12%, the concentration of the stacking gel was 5%, and the mixed standard protein with a molecular weight of 14.4-116kDa was used as a control. A single band was shown in PAGE with a molecular weight of 60 kDa. Adopt HPLC to carry out purity detection, chromatographic column is Elit C4 column (5 μm, 250mm×4.6mm, 300A), column temperature is 30 ℃, acetonitrile-water (10:90) is mobile phase, flow rate 0.75ml / min, detection wavelength 280nm, the result showed a single peak, and the protein purity was 100%.

[0020] The protein was tested for its anti-tumor activity. It was detected by the MTT method. When the drug concentration was 200 g / ml, it was effective against LOVO (human colon cancer cell line), A549 (human lung cancer cell line), and L1210 (mouse leukemia cell line). strain...

Embodiment 2

[0025] The anti-tumor active protein of Sea Dragon provided in this example has the appearance of white powder, and SDS-PAGE shows a single band with a molecular weight of 60 kDa. Purity detection was performed by HPLC, which showed a single peak, and the protein purity was 100%.

[0026] The MTT method was used to test the antitumor activity of the active protein obtained in the present embodiment. When the drug concentration was 0.2 mg / ml, the antitumor activity of the Jianhailong protein was effective against LOVO human colon cancer cell line), A549 (human lung cancer cell line), L1210 ( The inhibition rates of mouse leukemia cell line) and CCRF-CEM (human leukemia cell line) were 53.2%, 57.2%, 51.3%, and 58.4%, respectively.

Embodiment 3

[0028] The anti-tumor active protein of Sea Dragon provided in this example has the appearance of white powder, and SDS-PAGE shows a single band with a molecular weight of 60 kDa. The purity test was performed by HPLC, which showed a single peak. Protein purity is 100%.

[0029] The MTT method was used to test the antitumor activity of the active protein obtained in the present embodiment. When the drug concentration was 0.2 mg / ml, the antitumor activity of the Jianhailong protein was effective against LOVO human colon cancer cell line), A549 (human lung cancer cell line), L1210 ( The inhibition rates of mouse leukemia cell line) and CCRF-CEM (human leukemia cell line) were 71.2%, 56.2%, 54.3%, and 63.4%, respectively.

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Abstract

The invention discloses a syngnathus acus antineoplastic active protein in the medicine technology field, which is an active protein whose molecular weight is 60 kDa which is extracted through using syngnathus acus as raw material. The outer appearance of protein is white powder, SDS-PAGE shows a single strip, and HPLC shows a single peak. The syngnathus acus antineoplastic active protein which is provided by the invention has the characteristics of antineoplastic activity and low toxicity. The invention has the antineoplastic activity by being used individually or used through combining with other pharmacophysics, which is mainly used for the therapy or auxiliary therapy of various cancers, such as lung cancer, liver cancer, cervical cancer, colon cancer, and leukemia and the like, and has specially stronger habitation effect for LOVO human colon cancer cell line, A5489 (human lung cancer cell line), L1210 (mouse leukemia cell line) and CCRF-CEM (human leukemia cell line).

Description

technical field [0001] The present invention relates to a protein product in the technical field of medicine, in particular to a protein with antitumor activity in Sea Dragon. Background technique [0002] Syngnathus acus Linnaeus is a commonly used Chinese herbal medicine. It is recorded in the 2005 edition of "Pharmacopoeia of the People's Republic of China". Nocturnal emission, carbuncle and furuncle. [0003] After searching the literature of the prior art, it was found that Li Shimin et al. found in the in vitro anti-tumor activity research on sea dragon medicinal materials that the fat-soluble part of sea dragon has the effect of inhibiting the human cancer cell line KB cells. (Li Shimin, Wu Xiaodan, Zeng Su, et al. Study on anti-tumor activity of sea dragon in vitro[J]. Chinese Journal of Traditional Chinese Medicine, 2001, 26(3): 198-201), which disclosed that the lipid with anti-tumor activity in sea dragon Soluble ingredients do not involve water-soluble active p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435A61P35/00
Inventor 李晓波王梦月聂玉晓鞠培俊
Owner SHANGHAI JIAO TONG UNIV
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