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Method for continuously extracting alkaloids, flavone and polysaccharide active components from mulberry leaves

A technology of active ingredients and alkaloids, which is applied in the field of extracting biologically active ingredients, can solve the problems of difficult separation, low extraction rate, unreported structure and category, etc., and achieve the effect of fast separation rate and high content of ingredients

Inactive Publication Date: 2008-07-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these patents can only separate one or two active ingredients, and the other active ingredients have to be removed as impurities in the purification process, which not only causes a lot of waste of resources, but also leads to higher product costs.
Moreover, existing patents all use water as a solvent to extract alkaloids in mulberry leaves, and our research experiments show that the extraction rate of water to alkaloids is lower than that of a certain concentration of solvent, and the components in the water extract are complex, giving Subsequent separations posed difficulties
In addition, the general concept of the extracted mulberry leaf polysaccharide is general, and the analysis of its structure and category has not been reported yet.

Method used

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  • Method for continuously extracting alkaloids, flavone and polysaccharide active components from mulberry leaves

Examples

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example 1

[0016] Add 400 g of the pulverized mulberry leaf raw material into 4 L of 30% ethanol aqueous solution, reflux extraction at 95 ° C, repeat the extraction 3 times, each time for 30 min, and the extracts obtained three times are 2.9 L, 3.7 L, and 4.1 L respectively, and combine Extract, filtered. The filter residue was dried in the air for later use, and the ethanol in the resulting filtrate was evaporated to dryness to obtain an extract, and then 2 L of deionized water was added to the extract to dissolve it, and then left to stand for 2 hours, and then centrifuged. The supernatant was passed through 200 g of D4020 macroporous resin and adsorbed at 25° C. for 5 hours. Then first rinse the macroporous resin with 2L deionized water, then elute the macroporous resin with 60% aqueous ethanol solution of 1L, collect the ethanol eluate, and evaporate the eluate to dryness under reduced pressure to obtain 6.3g of extractum, wherein the total flavonoids The mass content is 31%.

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example 2

[0020] Add 400 g of the pulverized mulberry leaf raw material into 4 L of 70% ethanol aqueous solution, reflux extraction at 95 ° C for 3 times, each time for 30 min, and obtain three extracts of 2.8 L, 3.3 L, and 3.8 L respectively, combine the extracts and filter, The filter residue was dried in the air for later use, and the ethanol in the obtained filtrate was evaporated to dryness to obtain an extract, and then 4 L of distilled water was added to the extract, left to stand for 2 hours, and centrifuged. The supernatant was passed through 200 g of H-103 macroporous resin and adsorbed at 30°C. Then first rinse the macroporous resin with 2L of deionized water, then elute the macroporous resin with 60% acetone aqueous solution of 1L, and evaporate the eluent to dryness under reduced pressure to obtain 7.6g of medicinal extract, wherein the mass content of total flavonoids is 36%. .

[0021] The solution after flowing through the macroporous resin was adsorbed by 60g of 001×7 ...

example 3

[0024] Add 400 g of the pulverized mulberry leaf raw material into 4 L of 30% ethanol aqueous solution, reflux extraction at 95 ° C for 3 times, each time for 30 min, and obtain three extracts of 2.8 L, 3.5 L, and 4.2 L respectively. The extracts are combined and filtered, and the residue Dry it for later use, evaporate the ethanol in the resulting filtrate to dryness to obtain an extract, then add 4L of distilled water to the extract, let it stand for 2 hours, and centrifuge. The supernatant was passed through 200 g of D101 macroporous resin and adsorbed at 28°C. Then first rinse the macroporous resin with 2L deionized water, then elute the macroporous resin with 60% acetone aqueous solution of 1L, and evaporate the obtained eluent to dryness under reduced pressure to obtain 6.5g of extractum, wherein the mass content of total flavonoids was 37%.

[0025] The solution after flowing through the macroporous resin was adsorbed by 80g of 001×7×7 cation exchange resin at 25°C for...

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Abstract

The invention relates to a method of taking mulberry leaves as the raw materials to continuously extract the active ingredients of alkaloids, flavonoids and polysaccharides. The invention includes the following steps: the solvents of 20 percent to 90 percent ethanol or 10 percent to 90 percent acetone are selected for reflux extraction to obtain the filtrate and filter residue; the filtrate is concentrated and dissolved by adding water, the flavonoid compounds with the content of 30 percent to 50 percent are obtained by macroporous resin separation; the solution is treated by ion exchange resin separation to get the alkaloid compounds with the content of 40 percent to 60 percent after passes through the macroporous resin; the filter residue of the mulberry leaves is boiled and decolored by using the water, and the acid polysaccharide compounds with the content of 55 percent to 85 percent are obtained by 10 percent to 60 percent alcohol sedimentation. The invention continuously and efficiently separates three active ingredients of alkaloids, flavonoids and polysaccharides of the mulberry leaves by one-time feeding, all the steps are not disturbed mutually, the extraction rate is high, the process operation is simple, the cost is low, and the invention is conductive to industrial popularization and application.

Description

technical field [0001] The invention relates to a method for continuously extracting active components of alkaloids, flavonoids and polysaccharides from mulberry leaves, which belongs to the technology of extracting biologically active components from natural products. Background technique [0002] Mulberry leaf, also known as iron fan, is the leaf of Moraceae mulberry (Morus alba.L), which tastes bitter, sweet and cold, and is distributed in most parts of the country. Modern pharmacological studies have proved that mulberry leaves can inhibit the rise of blood sugar, and can prevent and treat diabetes. In addition, mulberry leaves also have the effects of dispelling wind and clearing heat, cooling blood and improving eyesight, with mild medicinal properties and no toxic side effects. It has been officially classified as "both food and medicine" by the Ministry of Health. [0003] Mulberry leaves contain a variety of active ingredients, including alkaloids, polysaccharides,...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61P3/10C07D311/30C07G5/00C07H1/08C08B37/00A61K127/00
Inventor 李淑芬贾冬冬杨鸿玲林江博田松江
Owner TIANJIN UNIV
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