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Method for extracting isoflavone from red clover

A technology of red clover and isoflavones, which is applied in the field of extracting isoflavones, can solve the problems of no benefit to human health, complex procedures, high cost, etc., and achieve the effects of reducing pollution, simple process and low cost

Inactive Publication Date: 2008-06-25
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the deficiencies that generally exist in the process are: most of the extracted isoflavone glycosides, and according to relevant foreign research reports, it is isoflavone aglycones that exert the medicinal effect, even if some patent applications extract isoflavone aglycones, It is also a method of extracting glycosides with an organic solvent first, and then hydrolyzing and converting them into aglycones, and the process is complicated.
A large amount of organic solvents are used in organic solvent extraction, which are flammable, explosive, and costly. The residual organic solvents in the prepared red clover extract are not beneficial to human health

Method used

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  • Method for extracting isoflavone from red clover
  • Method for extracting isoflavone from red clover

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take the whole red clover, crush it, heat and reflux extract twice with 85% ethanol solution containing 1mol / L hydrochloric acid, each time for 1.5 hours, combine the extracts, filter, and use the filtrate for later use.

[0027] The above filtrate was recovered with ethanol, concentrated, adjusted to neutral pH with sodium hydroxide solution, centrifuged, dissolved in sodium hydroxide solution and adjusted to pH 10, centrifuged, and the supernatant was set aside.

[0028] The above supernatant was kept warm at 40°C, and then microfiltration, ultrafiltration and nanofiltration were performed sequentially in a roll-type membrane separation device with an operating pressure of 0.15 MPa. The microfiltration membrane is polyvinylidene fluoride membrane with a pore size of 0.45 μm, the ultrafiltration membrane is a polysulfone membrane with a molecular weight cut-off of 5000KD, and the nanofiltration membrane is a polyethersulfone membrane with a molecular weight cut-off of 2...

Embodiment 2

[0032] Take the whole red clover, grind it, heat and reflux extract twice with 65% ethanol solution containing 0.5mol / L hydrochloric acid, each time for 2 hours, combine the extracts, filter, and use the filtrate for later use.

[0033] The above filtrate was recovered with ethanol, concentrated, adjusted to neutral pH with sodium hydroxide solution, centrifuged, dissolved in sodium hydroxide solution and adjusted to pH 9, centrifuged, and the supernatant was set aside.

[0034] The above supernatant was kept warm at 30°C, and then microfiltration, ultrafiltration and nanofiltration were performed sequentially in a roll-type membrane separation device with an operating pressure of 0.10 MPa. The microfiltration membrane is polyvinylidene fluoride membrane with a pore size of 0.2 μm, the ultrafiltration membrane is a polyethersulfone membrane with a molecular weight cut-off of 1000KD, and the nanofiltration membrane is a polysulfone membrane with a molecular weight cut-off of 100...

Embodiment 3

[0038] Take the whole red clover, crush it, heat and reflux extract twice with 95% ethanol solution containing 0.1 mol / L hydrochloric acid, each time for 1 hour, combine the extracts, filter, and use the filtrate for later use.

[0039] The above filtrate was recovered with ethanol, concentrated, adjusted to neutral pH with sodium hydroxide solution, centrifuged, dissolved in sodium hydroxide solution and adjusted to pH 11, centrifuged, and the supernatant was set aside.

[0040]The above supernatant was kept warm at 25°C, and then microfiltration, ultrafiltration and nanofiltration were performed sequentially in a roll-type membrane separation device, with an operating pressure of 0.12MPa. The microfiltration membrane is polyvinylidene fluoride membrane with a pore size of 0.2 μm, the ultrafiltration membrane is polyethersulfone with a molecular weight cut-off of 50,000KD, and the nanofiltration membrane is polysulfone membrane with a molecular weight cut-off of 300KD.

[004...

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Abstract

The invention relates to a method for extracting the isoflavone from the red clover. The invention can obtain the high-purity isoflavone products through the steps of one-step hydrolysis method, water precipitation base-dissolving method, membrane separation, and column chromatography, etc. The invention carries out the separation and purification by adopting the membrane separation technology, thus on one hand enhancing the purity of the isoflavone glycosides in the products and on the other hand driving the sample solution before the macroporous resin column chromatography to be purified, lightening the damage of the resin and extending the using period of the resin. Furthermore, the application of the nanofiltration technology drives the sample solution before the resin column chromatography to be directly sampled without needing to be concentrated so as to simplify the working procedure. The process is simple, can be suitable for the industrialized production, and the edible grade ethanols are only adopted as the solvents in the process of preparation, thus causing few contaminations and requiring low cost.

Description

technical field [0001] The invention relates to a method for extracting isoflavones from red clover. Background technique [0002] Red clover (Trifolium pratense L.) is a perennial herb of the leguminous genus Clover, also known as red clover, red clover, red sage, gorse, and clover. In addition to being used as high-quality feed and pasture, the decoction of the inflorescence is also used by foreign folks for expectorant, diuretic and anti-inflammatory, to treat colds and tuberculosis, and to treat abscesses, burns and eye diseases with external application. , roots or whole plants are made into extracts, pastes, decoctions or tea for drinking to treat gastric ulcer, gastric cancer, breast cancer and intestinal cancer. The folks in our country use the inflorescences and flowering branches and leaves of red clover as medicine for antispasmodic, cough, and asthma. In addition, the whole plant can also be made into ointment to treat local ulcers. [0003] In recent years, th...

Claims

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Application Information

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IPC IPC(8): C07D311/36A61K36/48A61K133/00
Inventor 陈娟师彦平刘勇
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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