Feed-stuff additive for improving meat quality of avian fed with microorganism and its preparation method
A feed additive and microbial breeding technology, which is applied in animal feed, animal feed, plant raw materials, etc., can solve the problems of reducing antibiotic drug sensitivity, adolescent precocious puberty, increasing labor and breeding costs, and unsafe human health. Cellular immunity, repairing the body's response mechanism, and improving the effect of disease-resistant immunity
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Embodiment 1
[0042] The microbial poultry meat quality-optimized feed additive and its production method use bacteria strains that are optimized through screening and adopt a two-stage fermentation process, which includes the following steps:
[0043] The first step, the breeding and production of strains:
[0044] (1) Bacillus licheniformis, Lactobacillus plantarum, and Lactobacillus casei provided by the Culture Preservation Center of the Ministry of Agriculture are used to subculture through nutrient agar medium, and Saccharomyces cerevisiae is subcultured through starch medium to select and breed fast growth and strong colonies For strains with obvious characteristics, the strains should be preserved at a temperature of 0°C-4°C;
[0045] Nutrient agar medium: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, water 1000ml, pH7.0-7.2, sterilized at 121°C for 20min;
[0046] Starch medium: peptone 10g, NaCl 5g, beef extract 5g, soluble starch 2g, distilled water 1000ml, agar 15-20g, st...
Embodiment 2
[0062] The first step. Breeding and production of strains: same as Example 1;
[0063] Step 2. First Stage Fermentation
[0064] (1), Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus casei, Saccharomyces cerevisiae are respectively inoculated in the culture medium through steam sterilization according to the ratio of 40%, 15%, 10%, 35%, stirring speed 220r / min, the culture temperature was 35°C, the ventilation rate was 1:0.6, and after 24 hours of fermentation time, the compound bacterial agent expansion culture solution was obtained;
[0065] Weigh the culture medium according to the following weight percentage:
[0066] 2.5% soybean meal powder, 0.3% urea, 0.8% ammonium sulfate, 2.3% yeast powder, 1.2% corn steep liquor,
[0067] Corn flour 3.5%, molasses 1.5%, sodium carbonate 0.1%, magnesium phosphate 0.7%, sterile water 87.1%;
[0068] (2), inoculate the compound bacterial agent expansion culture solution in the substratum, the ratio is: strain expansion...
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