Method for producing fuel ethanol by secondary fermentation after kudzu root steam explosion pretreatment
A fuel ethanol and pretreatment technology, applied in the direction of microorganism-based methods, biofuels, biochemical equipment and methods, etc., can solve the problems of high consumption, cooking, liquefaction, etc., and achieve the goal of reducing energy consumption, shortening the cycle, and reducing production costs Effect
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Embodiment 1
[0019] Embodiment 1: get Radix Puerariae 50g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.5Mpa; In the material after processing, add glucoamylase (50U / g steam explosion dry material), at 60 After saccharification in a water bath for 30 minutes at ℃, cool to 30 ℃ and sterilize with ultraviolet light for 20 minutes. Under sterile conditions, add 0.1% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 and 0.10% activated yeast, and then carry out anaerobic fermentation at 30° C. for 60 h, and collect ethanol by distillation. Obtain 0.2217g ethanol / g steam explosion dry material, 4.7689g ethanol / 100g fermentation product.
Embodiment 2
[0020] Embodiment 2: Get Radix Puerariae 50g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.6Mpa; In the material after processing, add glucoamylase (80U / g steam explosion dry material), at 55 After saccharification in a water bath for 60 minutes at ℃, cool to 32 ℃ and sterilize with ultraviolet light for 15 minutes. Under sterile conditions, add 0.05% (NH 4 ) 2 SO 4 , 0.1% KH 2 PO 4 And 0.25% of the activated yeast, followed by anaerobic fermentation at 32° C. for 54 hours, and ethanol was collected by distillation. Obtain 0.4057g ethanol / g steam explosion dry material, 6.6423g ethanol / 100g fermentation product.
Embodiment 3
[0021] Embodiment 3: get Radix Puerariae 50g, cut into small sections, drop into steam explosion tank, process 2.5 minutes under steam pressure 0.7Mpa; After saccharification in a water bath for 40 minutes at ℃, cool to 35 ℃ and sterilize with ultraviolet light for 18 minutes. Under sterile conditions, add 0.1% (NH 4 ) 2 SO 4 , 0.05% KH 2 PO 4 and 0.30% activated yeast, and then carry out anaerobic fermentation at 35° C. for 48 hours, and collect ethanol by distillation. Obtain 0.4205g ethanol / g steam explosion dry material, 7.6039g ethanol / 100g fermentation product.
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