Method for establishing high-efficiency somatic cell embryogenesis regeneration plant of plantain banana and Brazilian banana

Abstract

The invention discloses a method for establishing high-efficiency somatic embryogenesis regeneration plants of plantain and braziliana. In this method, before the induction of somatic embryos with suspension cells, they are pre-cultured with M2 liquid medium without 2,4-D for 7 to 10 days, and the cell clusters with a relatively consistent size and growth state are screened out through a sieve, and inoculated into somatic embryos. on the induction medium. Pre-cultivation can increase the induction rate of somatic embryos and the subsequent plant transformation rate by about 1 times; the cell clusters with a size ranging from 154 μm to 900 μm obtained through sieves have the best induction effect of somatic embryos; The optimum inoculum volumes were 0.4ml 20% SCV and 1ml 20% SCV respectively. In addition, adding ABA and ascorbic acid on the prior art somatic embryo induction medium of bananas at the initial stage of somatic embryo induction prevents to a certain extent the phenomenon that plantains are prone to browning during somatic embryo induction, and can effectively improve plantain and Frequency of somatic embryogenesis in embryogenic suspension cells of Banana brasiliensis.

Description

technical field [0001] The invention belongs to the field of banana biotechnology breeding, in particular to a method for effectively increasing the frequency of somatic embryogenesis of plantain (MusaABB paradisiaca Linn.cv.Da Jiao) and Brazilian plantain (Musa AAA Cavendish cv.Baxi) embryogenic suspension cells (ECS) Methods for successfully regenerating plants. Background technique [0002] Banana (including plantain) is one of the most important economic crops and crops in the world, and is also the main food in many tropical and subtropical countries and regions. It provides staple food for nearly 500 million people and is the fourth largest food after rice, wheat and corn. source of food. The total output of bananas in my country ranks third / fourth in the world, and they are widely planted in Guangdong, Fujian, Hainan, Guangxi, and Yunnan provinces. The production and sales volume has always been the top among southern fruits, and it is known as one of the four major ...

AI Technical Summary

Problems solved by technology

However, there are deficiencies in this culture system: the collection of inflorescence explants is limited by the budding season, the difference in the physiological state of inflorescences of different plants, the difference in climate when samples are collected, and many other factors affect the stability of the system, resulting in The induction rate of embryogenic callus is usually lower than 4%; the conversion efficiency of embryogenic callus to ideal ECS and its somatic embryogenesis ability and plant conversion rate (usually not higher than 20%) are very low

In view of the above technical bottlenecks, there are no reports on the establishment of Brazilian plantain ECS and plantain ECS and the regeneration of plants through somatic embryogenesis.

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