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Method for producing L-lactic acid and coagulate bacillus cereus special for the same

A technology of Bacillus coagulans and lactic acid, which is applied in the field of producing L-lactic acid, can solve the problems of low acid production rate, achieve the effects of less chance of polluting miscellaneous bacteria, reduce the loss of nutrients, and reduce the consumption of energy

Active Publication Date: 2008-05-07
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patents USP7183088 B2 and CN1498265 A mentioned that another strain of Bacillus coagulans (Bacillus coagulansSIM-7 DSM14043) was used to produce L-lactic acid, but the rate of acid production was low. After 98 hours of fermentation, the acid production was 12.3%, and the conversion rate was 95.5%.

Method used

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  • Method for producing L-lactic acid and coagulate bacillus cereus special for the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Isolation, mutagenesis, screening and identification of Bacillus coagulans CASH CGMCC № 2184

[0035] 1. Isolation, mutagenesis and screening

[0036] The composition of the medium used in this example is as follows:

[0037] Nutrient liquid medium: glucose 150 g / liter, yeast powder 10 g / liter, calcium carbonate 50 g / liter, pH 6.

[0038] Nutrient agar medium: glucose 50g / L, yeast powder 10g / L, calcium carbonate 20g / L, agar powder 10g / L, pH 6.

[0039] Fermentation medium: glucose 150 g / liter, yeast powder 20 g / liter, calcium carbonate 90 g / liter, pH 6.

[0040] Nutrient broth medium: glucose 50 g / l, yeast powder 10 g / l, calcium carbonate 10 g / l, pH 6.

[0041] The specific operation process of this embodiment is as follows:

[0042] A soil sample was obtained from near a milk factory in Mentougou District, Beijing, and every 2 grams of soil sample was put into 50ml nutrient liquid medium, and enriched and cultured at 55°C for 6-10 hours. Then, it was diluted and s...

Embodiment 2

[0047] Example 2: Using Bacillus coagulans CASH CGMCC №2184 to ferment to produce L-lactic acid in a triangular flask

[0048] The composition of the medium used in this example is as follows:

[0049] Slant medium: glucose 20g / l, yeast extract 5g / l, peptone 10g / l, beef extract 10g / l, magnesium sulfate heptahydrate 0.58g / l, manganese sulfate tetrahydrate 0.25g / l, carbonic acid Calcium 15g / L, agar powder 15g / L. The initial pH of the medium was 6.5. Sterilized at 115°C for 20 minutes.

[0050] Seed culture medium: glucose 70 g / l, yeast powder 10 g / l, soy peptone 5 g / l, calcium carbonate 30 g / l. The initial pH of the medium was 6.5. Sterilized at 115°C for 20 minutes.

[0051] Fermentation medium: glucose (Zibo Hongyu Industrial Sugar Co., Ltd.) 170 g / liter, yeast powder (Beijing Aoboxing Biotechnology Co., Ltd., catalog number 01-013) 15 g / liter, calcium carbonate 80 g / liter. The initial pH of the fermentation medium was 6.5. Not sterilized.

[0052] The method for producing L-lacti...

Embodiment 3

[0063] Example 3. Using Bacillus coagulans CASH CGMCC №2184 to ferment to produce L-lactic acid in a triangular flask

[0064] The culture temperature of Bacillus coagulans CASH CGMCC №2184 is 55°C. The composition of the fermentation medium is: glucose (Zibo Hongyu Industrial Sugar Co., Ltd.) 118 g / l, soy peptone (Beijing Aoboxing Biotechnology Co., Ltd., catalog number 01-004) 15 g / l, calcium carbonate 80 g / Rise. The initial pH of the fermentation medium was 5.5. The fermentation time is 24 hours. The other methods are the same as in Example 2.

[0065] At the end of fermentation, the concentration of glucose is 3.2 g / liter ± 1.1 g / liter (mean ± standard deviation), the concentration of L-lactic acid is 113.5 g / liter ± 1.9 g / liter (mean ± standard deviation), and the sugar-acid conversion rate is 98.9 %±0.3% (average value±standard deviation), volume production efficiency 4.7 g / l / hr±0.2 g / liter / hr (average value±standard deviation), L-lactic acid optical purity 99.3%±0.2% (aver...

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Abstract

The invention discloses a production method of L-lactic acid and the special condensate bacillus CASH CGMCC No. 2184. L-lactic acid can be gotten by cultivating the special condensate bacillus CASH CGMCC No. 2184. Per litter of fermentation culture medium of the condensate bacillus comprises at least one of the following five nitrogen sources: yeast powder 5 to 15g, peptone 5 to 15g, soybean peptide 5 to 15g, soybean peptone 5 to 15g, and cottonseed protein 10 to 20g; in addition, the fermentation culture medium comprises glucose 80 to 170g (the content of initial glucose), calcium carbonate 50 to 100g and water; the pH of the fermentation culture medium is 5.5 to 7. With glucose as the substrate, under the condition of 45 to 60DEG C and with 98 to 99% of sugar acid conversion, the condensate bacillus CASH CGMCC No. 2184 efficiently ferments to produce L-lactic acid with optical purity of 99%; the concentration of the L-lactic acid maximum can attain 190 g / l and the volume production efficiency maximum can attain 5.7 g / l / h.

Description

Technical field [0001] The invention relates to a method for producing L-lactic acid and its special Bacillus coagulans. Background technique [0002] Lactic acid, also known as dihydroxypropionic acid, is one of the world's three major organic acids and is widely used in the food, cosmetics, pharmaceutical, textile and chemical industries. Polylactic acid made from lactic acid has good biocompatibility and biodegradability, and can be made into medical sustained-release materials, biodegradable fibers and biodegradable plastics, and is expected to replace petroleum-based plastics in the near future. In recent years, due to the increasing shortage of petroleum resources and the increasing awareness of people's environmental protection, there are more and more researches on polylactic acid, and the demand for lactic acid as a monomer of polylactic acid is becoming more and more urgent. Lactic acid is a chiral molecule, which can be divided into L-lactic acid, D-lactic acid and DL-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/07
Inventor 许平秦加阳赵博马翠卿于波
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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