Two-dimensional array liquid phase chromatogram-mass spectrometry combination method
A liquid chromatography and two-dimensional array technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of separation time limitation and separation ability, and achieve the effect of simple structure, increased separation ability and wide application range
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Embodiment 1
[0020] Two-dimensional array liquid chromatography-mass spectrometry was used to analyze the chloroform extraction parts of the methanol extract of traditional Chinese medicine Yinchen.
[0021] In this experiment, CN column (250×4.6mm) was used for the first dimension, and C18 column (150×2.1mm) was used for the second dimension, and gradient elution of acetonitrile (B) and water-0.5% formic acid (A) was used for both dimensions. Due to the limited laboratory hardware, the offline "1+4" mode 2D-Array-HPLC / MS is currently used. That is, one cyano column is used in the first dimension, and four C18 columns are used in the second dimension. The chromatographic condition of the first dimension is that 15% B increases linearly to 75% B within 72 minutes, the flow rate is 1 mL / min, and the detection wavelength is 254 nm. The chromatographic condition of the second dimension is that 15% B rises linearly to 39% B within 20min, then linearly rises to 60%B within 30min, and finally ri...
Embodiment 2
[0026] Two-dimensional array liquid chromatography-mass spectrometry was used to analyze the n-butanol extraction parts of the methanol extract of traditional Chinese medicine Yinchen.
[0027]The same chromatographic system as that used in Example 1 was used to analyze the n-butanol part of the traditional Chinese medicine Capillary chinensis. The chromatographic condition of the first dimension cyano column is that 10% B rises linearly to 18% B in 16 minutes, then rises linearly to 45% B in 5 minutes, and then rises linearly to 100% B in 4 minutes, and the flow rate is 1mL / min , the detection wavelength is 254nm. The chromatographic conditions of the second dimension C18 column are 10% B linearly increased to 25% B within 18min, then linearly increased to 32%B within 14min, and then linearly increased to 65%B within 33min, the flow rate was 0.2mL / min , using full-wavelength scanning, the monitoring wavelength is 254nm. The mass spectrometry conditions of the second dimensi...
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