Micro fluid control array protein chip and its usage method

A protein chip and microfluidic technology, applied in the field of protein chips, can solve the problems of insufficient operation and low degree of automation, and achieve the effects of reducing production costs, easy operation, and rapid analysis

Active Publication Date: 2008-04-23
HANGZHOU JINCHENGYIBANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent document with the publication number CN1635146 discloses a one-dimensional microfluidic array protein chip, which irreversibly bonds the microfluidic channel to the chip substrate, and has the advantages of extremely small amount of sample, not easy to pollute, high throughput, quantitative However, the microchannel of the protein chip requires a special structure (that is, the positive mold also needs to be specially prepared), and the capture probe needs to be modified by special particles, and it still needs to be sampled and assembled; therefore, the operation is not easy and the degree of automation is not high.

Method used

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  • Micro fluid control array protein chip and its usage method
  • Micro fluid control array protein chip and its usage method
  • Micro fluid control array protein chip and its usage method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Using a silicon wafer with photoetched patterns as a positive mold and polydimethylsiloxane (PDMS) as a substrate, fabricate 20 lateral microfluidic channels (channel width 100 microns, thickness 3mm) The PDMS substrate, that is, the microfluidic channel sheet; after the PDMS substrate is peeled off from the mold, use a puncher (diameter 1mm) to punch a hole (diameter 0.6-0.8mm) at the end mark of the microchannel as the inlet and outlet These holes are used to insert microfluidic tubing, such as inlet and outlet capillaries, into the microchannel.

[0031] 2. Two pieces are processed by conventional methods (any one of them contains a microfluidic pipeline channel, which is used for the passage of liquid inlet and outlet thin tubes, and the position is the same as figure 1 The inlet and outlet holes of the middle PDMS channel sheet correspond to each other. ) plexiglass as a clip, reversibly seal the PDMS substrate containing the transverse microchannel and the sil...

Embodiment 2

[0034] 1. Using a silicon wafer with photoetched patterns as a positive mold and polydimethylsiloxane (PDMS) as a substrate, fabricate 50 longitudinal microfluidic channels (channel width 50 microns, thickness 5 mm) PDMS substrate; after the PDMS substrate is peeled off from the mold, holes are punched at the end mark of the microchannel with a puncher.

[0035] 2, by the method similar in embodiment 1 step 2, use two pieces of processed plexiglass as clips, the PDMS substrate containing the longitudinal microchannel and the gold-plated glass slide (manufacturer, GENEFLUIDICS company product) as the chip substrate Perform reversible sealing, and connect the chip to a microinjection pump through a microplastic tube.

[0036] 3. Prepare 9 target protein nucleic acid aptamer assembly solutions No. 1-9 (concentrations: 1×10 -10 M, 5×10 -10 M, 1×10 -9 M, 5×10 -9 M, 1×10 -8 M, 5×10 -8 M, 1×10 -7 M, 5×10 -7 M, 1×10 -6 M, the nucleic acid aptamer is Shanghai Sangon Bioenginee...

Embodiment 3

[0038] 1. Prepare 20 PDMS substrates with longitudinal microfluidic channels (100 microns in channel width and 7 mm in thickness) according to the method of step 1 in Example 1, and combine the PDMS substrate with the longitudinal microchannels with the immunoglobulin obtained in Example 1 The protein-assembled chip is reversibly sealed, and the chip is connected to a micro-injection pump through a micro-plastic tube. Such as figure 1 As shown, the two layers of microchannels assembled on the microfluidic array protein chip are vertically crossed in the central area of ​​the chip.

[0039] 2, 9 groups of concentration (10nM) identical sample solutions (goat anti-mouse immunoglobulins on the surface of gold nanoparticles) containing goat anti-mouse immunoglobulin (manufacturer, U.S. FITZGERALD company product) of 15nm nano-gold markers Assembled, the method refers to Nam, JM etc., "Science" volume 301 page 1884) at a speed of 2 microliters / hour through the microfluidic channel...

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Abstract

Said protein chip comprises a micro flow control channel layer and a chip substrate; at least one of both above is made of soft substance. It features: the micro flow control layer comprises two layers respectively having a first micro flow control channel and a second micro flow control channel; the first micro flow control channel is used for assembling the capturing probes; the second micro flow control channel is used for adding the tested protein sampler and the detection probes; said first micro flow channel layer and the second micro flow control channel layer are located on the chip substrate and forms 2D micro-array pattern on the chip.

Description

technical field [0001] The invention relates to the field of protein chips, in particular to a microfluidic array protein chip with a two-dimensional structure and an application method thereof. Background technique [0002] With the development of proteomics and molecular diagnostics, as an urgently needed miniaturized, integrated, high-throughput, fast and efficient protein analysis technology, protein chip has attracted extensive and deep attention since its birth. In proteomics research, it can replace traditional research methods such as two-dimensional electrophoresis and double yeast hybridization, so as to reduce time, reduce costs and improve accuracy, and obtain rich experimental data, making omics research even more powerful and realizing Fast forward. In terms of molecular diagnostics, it only needs a small amount of samples (microliters or even nanoliters) to realize the simultaneous analysis of multiple disease markers, which can be used for early diagnosis of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N35/00
Inventor 宋世平樊春海颜娟
Owner HANGZHOU JINCHENGYIBANG TECH CO LTD
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