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Method for determining quality of inosine tablets

A measurement method, the technology of inosine tablets, applied in the direction of measuring devices, electrochemical variables of materials, instruments, etc., can solve the problems of difficult to eliminate interference, high test cost, expensive equipment, etc., achieve short reaction time, reduce cost, and simple method Effect

Inactive Publication Date: 2008-04-16
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, hypoxanthine and inosine have a common ultraviolet absorption structure, so it is difficult to exclude the interference of inosine analogues such as hypoxanthine by using the ultraviolet spectrophotometric method in the determination of inosine content in inosine tablets.
It is reported that high pressure liquid chromatography is used to determine the content of inosine in inosine tablets. Although the separation effect is good, the required equipment is expensive and the test cost is high, so it is difficult to popularize and apply.

Method used

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  • Method for determining quality of inosine tablets
  • Method for determining quality of inosine tablets
  • Method for determining quality of inosine tablets

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Embodiment Construction

[0028] 1 Instruments and reagents: nucleoside phosphorylase (EC 2.4.2.1), xanthine dehydrogenase (EC 1.2.3.2), inosine reference substance Sigma Company of the United States; bovine serum albumin, glutaraldehyde Shanghai chemical reagent purchase Imported sub-packaging at the supply station; nuclear microporous membrane (0.2 μm) from Nucleopore Company of the United States; platinum and silver purity of 99.999%, Jinan Branch of the People's Bank of China; other reagents are analytically pure. SBA-40 inosine biosensing analyzer.

[0029] 2 Determination principle: The determination of inosine by immobilized double-enzyme composite enzyme membrane combined with hydrogen peroxide electrode is based on the following reaction:

[0030]

[0031]

[0032]

[0033]

[0034] 3 Preparation of inosine reference substance solution: Accurately weigh 268 mg of inosine reference substance, put it in a 1000ml volumetric flask, add distilled water to dissolve, and const...

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Abstract

The invention relates to a measuring method of the quality of inosine and belongs to the biosensor technical field. The preparing method is that: nucleoside phosphorylase and hypoxanthine dehydrogenase are co-immobilized and combined with the electrode of H202 to form an inosine enzyme electrode biosensor; phosphate buffer solution of 0.1 mmol.L< -1 >PH7.2 is adopted; the amount of hypoxanthine contained in the standard solution is 268mg.L< -1 >; 25ul standard solution is injected into a reaction tank for 25 seconds, then the instrument automatically shows the base liveness of enzyme membrane; a calibrate key is pressed to calibrate 100, and the sample can be measured after automatic washing.

Description

[0001] Field [0002] The invention relates to a drug analysis and determination method. Specifically, the present invention relates to a method for determining the main effective components of inosine tablets. Background technique [0003] Inosine is a normal component in the human body. It participates in nucleic acid metabolism, energy metabolism and protein synthesis in the human body, activates the pyruvate oxidase system, increases the activity of coenzyme A, and enables tissue cells in low-energy and hypoxic conditions to continue to metabolize. It can also improve liver function, help the recovery of damaged liver cell function, stimulate the body to produce antibodies, and improve intestinal absorption of iron. [0004] Inosine tablets are coenzyme drugs that can improve the metabolism of the body. For various types of liver disease, heart disease, leukopenia, thrombocytopenia, central retinitis, optic atrophy, etc. are national essential drugs. [0005] Medicinal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/49G01N27/327
Inventor 孙士青史建国杨俊惠马耀宏李雪梅张立群孟庆军杨艳王丙莲
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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