Method for removing fermentation inhibitor and pigments of sugar solution

A technology of fermentation inhibitors and sugar solution, which is applied in fermentation, biofuel, etc., can solve the problems of incomplete removal of impurities and high cost, and achieve the effects of low cost, good fermentation quality and product quality, and high purity

Active Publication Date: 2011-10-19
郑州运维生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings of the existing sugar liquid treatment methods, such as high cost, incomplete removal of impurities, and easy introduction of new substances to affect the progress of fermentation, the present invention provides a removal method with low cost, complete removal of impurities, and no introduction of new substances. A New Approach to Fermentation Inhibitors and Pigments in Sugar Liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] The mixed solution of sodium benzoate, furfural and glucose is 100ml, the mass concentration of the three substances is 1%, adjust its pH=2, add 5g of activated carbon, 0.5ml of hydrogen peroxide with a mass percentage of 30%, and react at 25°C Mix well, stir slowly in the middle, measure the concentration of each substance in the reaction at 1h, 2h, 4h, 8h, 16h, and 24h respectively, after testing, the removal rate of sodium benzoate reaches 100% after 2h of reaction, and the removal rate of furfural at 4h The removal rate can reach 100%, while the total reduction of glucose is only 10% until 8h. The reacted solution was filtered to remove the activated carbon, and the residual hydrogen peroxide was removed with catalase, and re-tested, the composition was single, and only glucose remained. The color of the solution changed from light yellow to nearly colorless, and the decolorization effect was good.

Embodiment 2

[0011] Mixed solution of sodium benzoate, furfural and glucose 100ml, wherein the mass concentration of the three substances is 1%, adjust its pH=4, add 5g of activated carbon to it, and pass through ozone so that the final concentration in the solution is 3g / m 3 , under the reaction conditions of 15°C, mix well and evenly, stir slowly in the middle, measure the concentration of each substance in the reaction at 1h, 2h, 4h, 8h, 16h, and 24h, and the removal rate of sodium benzoate reaches 80% after 2h of reaction %, the removal rate of furfural reached 100% at 4h, while the total reduction of glucose was only 10% until 8h. The reacted solution was filtered to remove the activated carbon, and the residual ozone was removed by stirring and standing for 1 hour, and retested, the composition was single, and only glucose remained. The color of the solution changed from light yellow to nearly colorless, and the decolorization effect was good.

Embodiment 3

[0013] Mixed solution 100ml of sodium benzoate, furfural and glucose, wherein the mass concentration of the three substances is 1%, adjust its pH=1, add 2g of activated carbon, 0.5ml of hydrogen peroxide with a mass percentage of 30% therein, feed ozone to make it The final concentration in the solution is 1g / m 3 , under the reaction conditions of 35°C, mix thoroughly and evenly, stir slowly in the middle, measure the concentration of each substance in the reaction at 1h, 2h, 4h, 8h, 16h, and 24h, and the removal rate of sodium benzoate reaches 100% after 1h of reaction %, the removal rate of furfural reached 100% at 2h, while the reduction of glucose was only 10% at 5h. The reacted solution was filtered to remove the activated carbon, the residual hydrogen peroxide was removed with catalase, and the residual ozone was removed by stirring and standing for 1 hour. Re-tested, the composition was single, and only glucose remained. The color of the solution changed from light yel...

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PUM

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Abstract

The invention discloses a method for removing a fermentation inhibitor and pigments of a sugar solution by using oxidative degradation and adsorption, which belongs to the field of fermentation industries. Aiming at the defects that the detoxification cost is high, impurities are incompletely removed, and the fermentation is affected by new matters which are most likely to be introduced, and the like in the traditional sugar solution, the method disclosed by the invention is used for carrying out oxidative degradation and adsorption through the combined effect of activated carbon / hydrogen peroxide / ozone so as to remove the fermentation inhibitor and the pigments of the sugar solution; the method has the advantages that reaction conditions are mild, the processing effect is good, the removal rate of heterocyclic ring and benzene ring matters such as furaldehyde, hydroxymethylfurfural, and the like in the processed sugar solution reaches more than 90%, the removal rate of other inhibitors reaches more than 80%, pigment matters can be completely removed, the reduction rate of nutritional compositions is not higher than 15%, the numerous loss of the nutritional compositions in the sugar solution is reduced, and the method does not cause pollution to environments and is suitable for industrial popularization and application.

Description

technical field [0001] The invention relates to a method for removing fermentation inhibitors and pigments in sugar liquid, in particular to a method for removing fermentation inhibitors and pigments in sugar liquid by oxidative degradation and adsorption, and belongs to the field of fermentation industry. Background technique [0002] The raw materials of the fermentation industry are rich in sources, mainly using corn, wheat and other food crops. At present, new fermentation raw materials have been developed, such as corn straw, rice straw, xylose residue and other cellulose substances. These fermented raw materials are processed by different methods, and finally become fermented sugar liquid containing nutrients for fermentation, including acid hydrolysis, high-pressure hydrolysis, enzymatic hydrolysis and other treatment methods. However, in more severe treatment methods such as acid hydrolysis and high-pressure hydrolysis, various substances that affect fermentation wil...

Claims

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Application Information

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IPC IPC(8): C12P7/10
CPCY02E50/10
Inventor 吴健戴桂馥刘孝臣刘絮
Owner 郑州运维生物技术有限公司
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