Immobilized cell single-tank high-strength continuous fermentation process for succinic acid

Abstract

The invention discloses a single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid, which comprises the procedures including that: firstly, seed liquid of high-activity actinobacillus capable of producing succinic acid is prepared; secondly, the seeds are proliferated in cultivation with a10L pot; thirdly, the 10L-pot solified cells are prepared; fourthly, the repeated single-pot fermentation with the 10L pot is carried out, then the seeds are inoculated into a fresh substrate, the first batch of fermentation is controlled within 24 hours, followed by 9 batches of continuous fermentation, each of which lasts for 16 hours, then the amphoteric anaerobic cultivation pattern is controlled through the current velocity of the combined air of CO2 and H2, the pH of the fermentation liquid is controlled with fed-batch of calcium oxide, wherein, at the end of each batch of fermentation, the next batch of fermentation is carried out through intercepting the solidified cells with positive-pressure sterilized operation with a propeller filter arranged inside the pot, the fermentation intensity is stabilized above 3.10g / (h.L), and the single-pot high-intensity continuous fermentation technology used for solidified cells with succinic acid is completely accomplished.

Description

technical field [0001] The invention relates to a new technology for bacterial fermentation of succinic acid, more specifically a new technology for single-tank high-intensity continuous fermentation using immobilized cells of Actinobacillus succinogenes, belonging to the field of fermentation engineering. Background technique [0002] As an important organic acid, succinic acid is widely used in medicine, pesticides, dyes, spices, paints, food, plastics and photographic materials industries, and the greater potential market of succinic acid is because it can be used as an intermediate in chemical synthesis. At present, many chemical products use benzene as raw material, but benzene is a petrochemical product and is not renewable. In the long run, it is necessary to find suitable renewable raw materials to replace benzene. About 250 chemical products that can be produced from benzene can be produced from succinic acid. Once the large-scale production of succinic acid is re...

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Problems solved by technology

[0004] Among the various immobilization technologies, the adsorption and embedding of natural polymers and synthetic polymer materials is the most active method of microbial cell immobilization. However, there is no patent research report on the use of immobilized cells for succinic acid fermentation. This patent uses calcium alginate immobilized Actinobacillus succinogenes (ATCC55618) cells for continuous high-intensity fermentation of succinic acid, which is of great significance for industrial application

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