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Quick test strip for detecting hydrogen peroxide in food

A technology of hydrogen peroxide and test strips, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators, etc. It can solve the problems of poor stability of modified electrodes, complicated process, and unfavorable preservation of enzymes. Biological activity and other issues, to achieve the effect of shortening sample processing time, reducing production costs, and improving production efficiency

Inactive Publication Date: 2008-03-19
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of preparing the modified electrode by this method is quite complicated, and the thickness of the titanium dioxide gel film and the amount of adsorbed enzyme cannot be effectively controlled during the modification process, resulting in poor stability of the prepared modified electrode, and the gel film is not conducive to preservation. Due to the biological activity of the enzyme, the life of the modified electrode does not exceed 14 days, so the on-site rapid detection of hydrogen peroxide cannot be widely promoted

Method used

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  • Quick test strip for detecting hydrogen peroxide in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Figure 1 is a schematic diagram of the detection principle of the rapid detection of hydrogen peroxide test strips of the present invention, from which it can be seen that the change of the optical signal comes from the change of the color of the reaction substrate membrane of the test strip. It can be seen from FIG. 2 that the test strip for rapid detection of hydrogen peroxide of the present invention comprises an insulating waterproof substrate 1 , a reaction substrate membrane 2 , a filter layer 3 , a spacer 4 and a cover 5 . After the insulating and waterproof substrate 1 and the cover sheet 5 are superimposed, the hole is punched by mechanical punching method, the hole diameter is 0.3-0.4mm, and the hole wall is 2mm away from the edge of the substrate. The adsorption effect of the wall makes the sample evenly distributed in the sample hole, and the punching position should match the interface of the testing equipment. The round hole on the cover sheet 5 is the samp...

Embodiment 2

[0034] The test strip is composed of an insulating waterproof substrate 1, a reaction substrate membrane 2, a filter layer 3, a spacer 4 and a cover sheet 5. The insulating and waterproof substrate 1 is the base, and a round hole is arranged on the insulating and waterproof substrate 1. Cover the reaction area assembled into the test strip sequentially above the round hole in the order of the reaction substrate membrane 2 and the filter layer 3, cover the two ends of the reaction area with spacers 4, and the cover sheet 5 is arranged on the reaction substrate membrane 2, On the filter layer 3 and the spacer 4 , a round hole is arranged on the cover sheet 5 , which is aligned with the round hole on the insulating and waterproof substrate 1 .

[0035] The materials used for the insulating and waterproof substrate 1, filter layer 3, spacer 4 and cover sheet 5 of the test strip are the same as in Example 1, and the specific manufacturing process is as follows:

[0036] 1. The insu...

Embodiment 3

[0043] The materials used for the insulating and waterproof substrate, filter layer, spacer and cover sheet of the test strip are the same as in Example 1, and the specific manufacturing process is as follows:

[0044] 1. Insulation and waterproof base sheet and cover sheet are perforated. The punching method, aperture and requirements are the same as in Example 1.

[0045] 2. Fabrication of the reaction substrate film layer 2. The reagent formula adopts 0.285 g of chromogen, 0.001 mg / L of horseradish peroxidase, and the dosage and preparation method of other reagents are the same as in Example 1. Then the prepared reaction substrate film layer is mechanically cut into small pieces slightly larger than the round holes of the substrate, and covered on the small holes of the insulating and waterproof substrate 1 .

[0046] 3. Cut the glass fiber sheet into small pieces with the same size as the reaction substrate membrane 2 , and overlap and cover the reaction substrate membra...

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Abstract

The invention relates to a quick test strip used for detecting the hydrogen peroxide in food, and belongs to the biological detection technical field. The invention comprises an insulation waterproofing substrate, a reaction substrate substance membrane piece, a filtering layer, an isolation piece and a cover piece, wherein, the insulation waterproofing substrate adopts a foundation base, and round hole is arranged on the insulation waterproofing substrate, and the reaction area of the test strip is assembled on above the round hole according to the order of the reaction substrate substance membrane piece and the filtering layer and by covered in sequence, the isolation piece is covered on the two ends of the reaction area, and the cover piece is arranged above the reaction substrate substance membrane piece, the filtering layer and the isolation piece, a round hole is arranged on the cover piece, and the round hole is arrayed to the round hole on the insulation waterproofing substrate. Detecting the hydrogen peroxide by the test strip, the pre-treatment of the sample is simple, and the detection time is short, being generally less than 90 seconds; the detection limit reaches 0.5 ppm, the reproducibility is good, the effective preservation time of the test strip is one year, and the quick in-place detection of the hydrogen peroxide in the food can be realized.

Description

technical field [0001] The invention relates to a test strip in the technical field of biological detection, in particular to a rapid test strip for detecting hydrogen peroxide in food. Background technique [0002] At present, in order to kill microorganisms in food and increase the color pleasure of food, it is common for food manufacturers to add excessive or illegal oxidants or bleaching agents. Domestic food quality inspection reports show that excess hydrogen peroxide (hydrogen peroxide) remains in some processed bean products, noodle products, fish balls, salted duck, salted chicken, etc. Up to 1.5% hydrogen peroxide has also been detected in shark fins in the Hong Kong Special Administrative Region. The "China Food Sanitation Law" stipulates that there must be no hydrogen peroxide residues in food and health products. Hydrogen peroxide has toxic effects such as carcinogenicity, accelerated aging or induction of cardiovascular diseases. The current national standard...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 柴春彦刘国艳刘海峰
Owner SHANGHAI JIAO TONG UNIV
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