Nucleic acid automatic extracting instrument

Abstract

The present invention discloses an automatic nucleic acid extractor applied to the technical field of biology to extract nucleic acid, and the purpose of the present invention is to resolve the problem that processes, such as nucleic acid extraction, purification, semi-quantitativework, etc., cannot be fulfilled in one step. The mechanical parts include reserve part plates, an X-axis bracket (18), a Y-axis bracket (24), a second Y-direction slide plate (27), a first Y-direction slide plate (28), etc. The X-axis bracket (18) is horizontally fixed over a workbench (1) by means of upright columns and beams. The Y-axis bracket (24) is fixed on an X-axis slide plate (21), a Y-axis supporting arm (25) is vertical to an X-axis supporting plate (19), the second Y-direction slide plate (27) and the first Y-direction slide plate (28) are firmly integrated together by means of the reinforcing plate (29) of the Y-axis supporting arm and are nested on the Y-axis supporting arm (25) in the form of matching sliding connection. The reserve part plates, a waste liquid plate, a box cover plate, a reagent plate, a dilution plate, reaction plates and metal baths are fixed on the workbench (1). Stepper motors are connected with electric control parts via wires.

Description

technical field [0001] The present invention relates to a separation device used in the field of biotechnology, more specifically, to an automatic nucleic acid extractor. Background technique [0002] Nucleic acid isolation and purification are the first and most critical steps in gene amplification, nucleic acid sequence analysis, and gene hybridization. It can be said that the premise of successful nucleic acid testing is whether high-quality nucleic acid can be obtained from biological samples. Especially for biological samples under various conditions such as corruption, deterioration and pollution, the quality of nucleic acid extraction will directly affect the success or failure of nucleic acid testing. At present, the nucleic acid extraction techniques widely used in China are organic extraction method (phenol-chloroform extraction method), Chelex-100 method, CTAB method and silica bead method. However, these methods all have certain shortcomings: such as the experim...

AI Technical Summary

Problems solved by technology

At present, the nucleic acid extraction techniques widely used in China are organic extraction method (phenol-chloroform extraction method), Chelex-100 method, CTAB method and silica bead method, etc., but these methods all have certain deficiencies: such as the experimental operation time required for the extraction method Long, cumbersome operation steps, relying on manual operation and cannot be automated extraction, and these methods have high requirements on the operator's technical level and are likely to cause human pollution, which is far from meeting the requirements of high purity, high efficiency, and easy automation of nucleic acid extraction technology , not suitable for large-scale high-purity nucleic acid extraction

In addition, although the standardized nucleic acid-STR typing system has been basically established, and the efficiency of the amplification and result analysis steps in the nucleic acid testing process has been greatly improved, it is still difficult to extract, purify and quantify nucleic acids from various samples. As the speed-limiting step in the inspection process, there is no automatic nucleic acid extractor that can well meet the requirements of forensic nucleic acid inspection and can complete nucleic acid extraction, purification, and semi-quantitative operations in one step, and there are no relevant patents and technical standards

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