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Application of KILLER polypeptide in preparing medicine for treating tumor

A technology for tumors and medicines, applied in the field of preparation of medicines for treating tumors, can solve the problems of limited application, lack of tumor cell specificity, toxic side effects, insensitivity to chemotherapy and radiotherapy, and achieve the effect of inducing tumor cell apoptosis

Inactive Publication Date: 2008-03-19
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemotherapy and radiotherapy are still the first choice for the treatment of cancer. Although the two have achieved considerable curative effect on the treatment of tumors, they have relatively large toxic and side effects due to the lack of specificity to tumor cells, and some tumor cells have a certain effect on chemotherapy. and insensitivity to radiotherapy, thus limiting their clinical application to a large extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 KILLER induces apoptosis of human colon cancer cell HCT116

[0028] Acquisition of the KILLER gene: the full-length cDNA fragment of KILLER was amplified from the human kidney cDNA expression library by PCR, and the length was 1182 bp; it was cloned into the eukaryotic expression vector after confirmation by sequencing.

[0029]Human colon cancer cell HCT116 was provided by ATCC. The cell line was cultured using Macoy's 5A complete medium (containing 10% calf serum, 100 U / ml penicillin and 100 U / ml streptomycin) of GIBCO Company. The tumor cells were transfected with the plasmid expressing KILLER for 48 hours, the cells were collected and stained with PI, and the cell apoptosis was detected by flow cytometry.

[0030] The results show that the KILLER of the present invention can promote the increase of the number of apoptotic cells in human colon cancer (HCT116).

Embodiment 2

[0031] Example 2 Attenuated Salmonella typhimurium expressing KILLER can induce tumor cell apoptosis

[0032] The tumor cells were cultured in complete medium without resistance, and the cells were infected with 250 MOI (the ratio of the number of virus particles to the number of tested cells) expressing KILLER and negative control attenuated Salmonella typhimurium for 3 hours, and then treated with antibiotics and 100 μg / ml G418 complete medium to grow cells. The cells were infected at different time points, and the induction of tumor cell apoptosis by attenuated Salmonella typhimurium expressing KILLER was detected.

[0033] The results showed that the attenuated Salmonella typhimurium expressing KILLER could induce tumor cell apoptosis.

Embodiment 3

[0034] Example 3 Attenuated Salmonella typhimurium expressing KILLER can inhibit the growth of tumor cells in mice

[0035] Attenuated Salmonella typhimurium expressing KILLER and negative control were used to feed tumor-bearing mice orally, and at different time points, the effects of attenuated Salmonella typhimurium expressing KILLER on the growth of tumor cells in mice and the growth cycle of mice were detected. Impact.

[0036] The results showed that the attenuated Salmonella typhimurium expressing KILLER could inhibit the growth of tumor cells in tumor-bearing mice and prolong the growth cycle of tumor-bearing mice.

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PUM

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Abstract

The invention belongs to the biomedical field and relates to the application of KILLER polypeptide in the preparation of a drug for remedying the tumor. A KILLER gene encoding production is a TRAIL accepter of cell surface as well as a lower target gene of a transcription regulator p53. An over-expression of the KILLER can very effectively induce the apoptosis of tumor cells. Attenuated salmonella typhimurium can be used as a carrier to introduce the KILLER to express inside the tumor cells and kill the tumor cells. The development of a novel treatment drug which considers the salmonella with the KILLER as orally taken gene not only solves the problem of non-injection path of a protein polypeptide drug but also more importantly provides the tumor treatment with a safe, effective, peculiar and economical method.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of KILLER polypeptide in the preparation of medicaments for treating tumors. Background technique [0002] Tumor disease has now risen to be the No. 2 "killer" in the world, and its death toll is second only to cardiovascular disease. Over the past few years, domestic and foreign medical circles have gained a new understanding of the pathogenesis of tumor diseases on the basis of cells. Based on the further understanding of the pathogenesis of tumors, people use various approaches to research and develop drugs that can specifically and effectively kill tumor cells without toxicity to normal cells. At present, chemotherapy and radiotherapy are still the first choice for the treatment of cancer. Although the two have achieved considerable curative effect on the treatment of tumors, they have relatively large toxic and side effects due to the lack of specificity to tumor cel...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P35/00
CPCY02A50/30
Inventor 王树林任大明符薇朱向莹
Owner FUDAN UNIV
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