Method for producing biological diesel oil catalyzed by lipase

Abstract

The invention discloses a method for producing biomass diesel under the catalysis of lipase, pertaining to the technical field of production of biomass diesel from fatty acids. Jatropha oil and methyl acetate are taken as raw materials, and the jatropha oil is pre-processed first; then, biomass diesel is produced by reaction between the jatropha oil and methyl acetate under the catalysis of fatty acids, methyl acetate: jatropha oil (mass ratio) = 1: 1-2, the dosage of fatty acids is 10 to 20 wt percent of the dosage of jatropha oil; the lipase is of columnar candida lipase, pubescent putrid mycete lipase, Antarctic candida yeast lipase B or thermophil fungal lipase, or of an synthase achieved by mixing two of the lipases. According to the method, methyl acetate is involved in the transesterification to provide ester-base; the reaction is free of enzyme inactivation, and the subsequent operation is convenient; the jatropha oil is easy to get and the price is low; the preparation process is simple, the reaction condition is mild and the environment is not contaminated; the yield of effective constituent (i.e. fatty-acid methyl ester) is as high as 66.27 to 87.57 percent.

Description

technical field [0001] The technical solution of the present invention relates to the field of biodiesel preparation from fatty acids, in particular to a method for producing biodiesel catalyzed by lipase. Background technique [0002] Biodiesel is a clean and safe new fuel that can replace petrochemical diesel produced from renewable resources such as vegetable oil or animal oil. [0003] At present, the preparation of biodiesel is mainly by chemical method, that is, the transesterification reaction is carried out with animal and vegetable oils and low-carbon alcohols such as methanol or ethanol under the condition of acidic or basic catalyst and high temperature of 230-250°C. CN1580190, CN1696247, CN1556174 all disclose the method for preparing biodiesel with chemical method. The chemical method has the following disadvantages: the process is complicated, the raw material alcohol must be excessive, the subsequent process must have a corresponding alcohol recovery device, ...

AI Technical Summary

Problems solved by technology

The chemical method has the following disadvantages: the process is complicated, the raw material alcohol must be excessive, the subsequent process must have a corresponding alcohol recovery device, the energy consumption is high, the cost is high, the esterification product is difficult to recover, the production process has waste lye discharge, and the environment is seriously polluted

[0006] In the above-mentioned existing technologies for preparing biodiesel, there are two major defects in both chemical and biological methods: first, most of the short-chain alcohols are used to participate in the transesterification reaction, providing ester group

However, short-chain alcohols, especially methanol, are easy to inactivate enzymes, and the product glycerol produced after methanol participates in the reaction is difficult to separate, which brings great trouble to the subsequent operation of the reaction; vegetable oil

With the shortage of water resources and the successive reduction of arable land in our country, the biodiesel preparation technology using vegetable oil or edible oil obtained from competitive arable land as raw materials will be greatly restricted