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Method for producing natural blue pigment

A production method and technology for blue pigment, applied in the field of bioengineering, can solve the problems of low reaction rate, unable to prepare green, purple, brown, difficult to industrialized production, etc., to shorten the reaction time, save the production cost, and improve the health care function. Effect

Inactive Publication Date: 2008-01-16
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, with the continuous deepening of the research on the safety of synthetic pigments, it was found that many synthetic pigments are harmful to human health, so the development and application of natural food pigments have gained people's attention again.
However, among the many natural pigments, most of them are red and yellow, while blue pigments are very rare. Without blue pigments, green, purple, brown and other colors cannot be formulated, which cannot meet the needs of food coloring and coloring. Therefore, The research and development of natural blue pigment has become a new issue that needs to be solved urgently
[0004] Domestic research on this pigment started late, and the method of directly adding gardenia fruit powder to the culture medium was mostly used to produce natural pigments. The color of the obtained products was mostly grayish with purple, and the yield was low, so it was difficult to industrialize production
[0005] Later, some researchers improved the production method and adopted a two-step method (Sun Lijun et al., using a two-step method to produce natural gardenia blue pigment, Journal of Nanjing Agricultural University, 1994, 17(4): 98-101), first using β-glucose The intermediate product genipin is obtained by catalyzing the reaction of geniposide with glucosidase; then the natural gardenia blue pigment is obtained by reacting genipin and amino acid under appropriate conditions, and the obtained gardenia blue pigment has ideal color, but its reaction rate is low, which affects the natural gardenia blue pigment. its industrial production

Method used

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  • Method for producing natural blue pigment
  • Method for producing natural blue pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 , Aspergillus niger fermentation to obtain β-glucosidase

[0025] Seed medium formula (g / L): 20g soluble starch, 10g glucose, KH 2 PO 4 1g, MgSO 4 1g, NaNO 3 2g, yeast extract 5g, pH4.

[0026] Fermentation medium formula (g / L): KH 2 PO 4 2g, MgSO 4 ·7H 2 O 0.2g, (NH 4 ) 2 SO 4 1.6g, bran is 3% of the liquid content, pH6.

[0027] According to the formula of the seed medium, the seed culture is carried out in a 250ml Erlenmeyer flask, the filling volume is 25ml, and the inoculation volume is about 2.0×10 spores 8 , the rotation speed of the shaker was 200 rpm, the culture temperature was 30° C., and the culture time was 44 h.

[0028] According to the formula of the fermentation medium, the fermentation culture was carried out in a 250ml Erlenmeyer flask with a liquid volume of 15ml, an inoculation volume of 750μl, a shaker rotation speed of 200rpm, a culture temperature of 30°C, and a culture time of 72h.

[0029] The β-glucose enzyme liquid...

Embodiment 2

[0030] Example 2 , Aspergillus niger fermentation to obtain β-glucosidase

[0031] Seed medium formula (g / L): soluble starch 15g, glucose 8g, KH 2 PO 4 0.8g, MgSO 4 0.8g, NaNO 3 1.8g, yeast extract 4.5g, pH4.

[0032] Fermentation medium formula (g / L): KH 2 PO 4 1.8g, MgSO 4 ·7H 2 O 0.15g, (NH 4 ) 2 SO 4 1.3g, bran is 5% of the liquid content, pH7.5.

[0033] According to the formula of the seed medium, the seed culture is carried out in a 250ml Erlenmeyer flask, the filling volume is 25ml, and the inoculation volume is about 2.0×10 spores 8 , the rotation speed of the shaker was 200 rpm, the culture temperature was 30° C., and the culture time was 48 h.

[0034] According to the formula of the fermentation medium, the fermentation culture was carried out in a 250ml Erlenmeyer flask with a liquid volume of 15ml, an inoculation volume of 750μl, a shaker rotation speed of 200rpm, a culture temperature of 30°C, and a culture time of 74h.

[0035] The β-glucose...

Embodiment 3

[0036] Example 3 , the preparation of gardenia blue pigment

[0037] Prepare a geniposide solution with a weight ratio concentration of 0.05%, take 4ml, and add an appropriate amount of the enzyme solution obtained in Examples 1 and 2 and 100 μl each of the β-glucosidase (FLUKA company) derived from bitter almonds as shown in Table 1 , and selectively add a metalloenzyme promoter (according to the volume of the reaction solution, the amount per ml is 0.02-0.25μg), react at 50-60°C for 30-120min, take samples every 10min during the reaction, and measure it by HPLC method The content of geniposide (wherein the HPLC collection of illustrative plates before and after reaction of 2# sample is as shown in Figure 1A and 1B, all the other samples are basically the same, so omit), and calculate conversion rate (conversion rate=1-recorded geniposide content / initial geniposide content), the results are shown in Table 2.

[0038] Then add different amino acids to the reaction solution...

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Abstract

The invention provides a production method of a natural blue pigment, which comprises genipin prepared by reaction of beta-glucosidase catalyzing gargenoside, and natural gardenia blue pigment prepared by reaction between the genipin and amino acid in a proper condition. The production method is characterized in that metalloenzyme accelerator is added in the reaction of beta-glucosidase catalyzing gargenoside. Because the metalloenzyme accelerator is added in the reaction, the speed of enzymatic reaction can be accelerated and the reaction time is shortened, so that the production cost is saved, which is helpful for industrialized production. Meanwhile, metal ions added are all the trace elements that the human body needs, so the prepared pigment not only does not have the potential harm that the artificial pigment has, but oppositely has good health function, therefore the pigment can be applied in various fields like food industry.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a production method of natural blue pigment. Background technique [0002] Natural colors were used until the invention of synthetic colors. However, because synthetic pigments have many advantages such as bright color, strong tinting strength, high stability, low price, and production not affected by seasons, they quickly occupied most of the pigment market share shortly after their appearance. . [0003] However, with the deepening of the research on the safety of synthetic pigments, it was found that many synthetic pigments are harmful to human health, so the development and application of natural food pigments have gained people's attention again. However, among the many natural pigments, most of them are red and yellow, while blue pigments are very rare. Without blue pigments, green, purple, brown and other colors cannot be formulated, which cannot meet ...

Claims

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Application Information

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IPC IPC(8): C09B61/00C12P19/60
Inventor 宫衡宓女付水林孙海燕刘必成刘朋波刘刚徐佳杰
Owner EAST CHINA UNIV OF SCI & TECH
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