Method for preparation of novel subunit vaccine for treating porcine pseudorabies

Abstract

The present invention discloses preparation process of new subunit vaccine of pig's pseudo rabies. The preparation process includes: differentially centrifuging pseudo rabies low virulent strain (PRV-FB strain), suspending the virus in PBS, regulating the protein concentration of the viral antigen, adding Mega-10 lytic virus cyst membrane protein, centrifugally extracting cyst membrane protein, adding proper amount of ISCOM matrix, bagging and dialyzing to obtain PRV type subunit vaccine. The PRV type subunit vaccine is safe and effective, and may be used in preventing and controlling pig's pseudo rabies.

Description

Technical field [0001] The invention relates to a method for preparing a novel subunit vaccine of porcine pseudorabies. Background technique [0002] Pseudorabies is a comorbid infectious disease of a variety of animals. Pigs are its natural host. After the infected pigs recover, they become invisible infection. It mainly causes abortion, stillbirth or mummification of pregnant sows. Newborn piglets show neurological symptoms, paralysis, exhaustion and death. The mortality rate is almost 100%, the incidence rate of weaned piglets is 20%-40%, and the mortality rate is 10%-20%. This disease has become the main cause of the current breeder reproductive disorder syndrome, and it is obviously increasing year by year. Because adult pigs are mostly invisible infections, and long-term poisoning and detoxification become a potential source of infection, which brings great difficulties to the control and elimination of the disease. At present, the effective prevention of PR in various coun...

AI Technical Summary

Problems solved by technology

Because most of the adult pigs are latently infected, and become a potential source of infection due to long-term virus-carrying and shedding, it brings great difficulties to the control and elimination of the disease

At present, the effective prevention of PR in all countries in the world is mainly vaccine immunization. The existing porcine pseudorabies vaccines include oil emulsion inactivated vaccines, traditional attenuated vaccines and gene deletion vaccines. The shortcomings of these vaccines are: such as oil emulsion inactivated vaccines. The safety of the vaccine is good, but the immune effect is not ideal, the local side effects of the injection are large, and serological tests cannot distinguish the vaccine immune antibody from the virulent antibody; the attenuated vaccine has a good immune effect, but it cannot distinguish the vaccine immune antibody from the virulent antibody, and there is virulence The risk of returning to strong; the gene deletion attenuated vaccine has a good immune effect and can distinguish vaccine immune antibodies from virulent antibodies, but gene deletion vaccines may become virulent strains due to genetic recombination and other potential unsafe issues

Therefore, improving the existing vaccines has become a difficult problem for researchers in various countries. The research on the porcine pseudorabies subunit ISCOM vaccine has been carried out abroad. The storage period is not clear, which limits the large-scale production and clinical application of vaccines

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