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Efficient liquid-phase chromatography method for determing reserpine content in rauvolfia plants

A high-performance liquid chromatography and Rauwolfia technology, which is applied in the field of high-performance liquid chromatography for determining the content of reserpine, an active ingredient in Rauwolfia plants, can solve problems such as large errors and complicated operations.

Inactive Publication Date: 2007-11-14
饶高雄
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the content analysis of reserpine in Rauwolfia, and some reports use paper chromatography electrophoresis or cellulose chromatography to separate the reserpine color band and then use spectrophotometry to determine the content, which is cumbersome and has large errors

Method used

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  • Efficient liquid-phase chromatography method for determing reserpine content in rauvolfia plants
  • Efficient liquid-phase chromatography method for determing reserpine content in rauvolfia plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take the dried Rauwolfia medicinal material as a coarse powder, accurately weigh about 2g, put it in a 100ml round bottom flask, accurately add 50ml of methanol, weigh, extract in a water bath under reflux for 1 hour, let it cool, and make up the lost weight with methanol. After filtration, the filtrate was filtered through a 0.45um membrane to obtain the test solution.

[0034] The conditions of determination: select Luna C-18 column of Phenomenex company for use; With acetonitrile-methanol-0.01% triethylamine aqueous solution (45-10-45) as mobile phase, flow rate 1ml / min; Column temperature 40 ℃; Detection wavelength 268nm; The sample volume is 20ul. The measurement results are shown in Table 5.

[0035] Table 5 Rauwolfia research samples and determination results from different origins

[0036] sample

Embodiment 2

[0038] Get sample Y-3 and Y-4 and prepare need testing solution according to embodiment 1. Measuring conditions: change the mobile phase ratio to acetonitrile-methanol-0.01% triethyl gum aqueous solution (35-25-40), and the remaining conditions are the same as in Example 1. The measurement results are shown in Table 6.

[0039] Table 6 Rauwolfia research samples and determination results from different origins

[0040] sample

Embodiment 3

[0042] Get sample C-3 and C-4 and prepare need testing solution according to embodiment 1. Determination conditions: change the mobile phase to acetonitrile-methanol-0.01% triethylamine aqueous solution (40-20-40), and the remaining conditions are the same as in Example 1. The measurement results are shown in Table 7.

[0043] Table 7 Rauwolfia research samples and determination results from different origins

[0044] sample

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Abstract

The invention provides a high-performance liquid chromatography method to determine reserpine quantity in rauvolfia genus. Characteristics: (1) column 18 to carbon alkyl bonded to silica fillers; (2) mobile phase is mixed solventcomposed of acetonitrile, methanol, 0.01% triethylamine, the volume ratio is acetonitrile 45% - 35%, methanol 10% - 25% triethylamine aqueous 45% - 40%, (3) detection wavelength 268 nm, column temperature was 40 deg.C , flow rate 1 ml / min, the sample size of 20 ul. The invention has been system comparative studied, and establish the high-performance liquid chromatography method to determine reserpine quantity in rauvolfia genus for the first time,which is accurate, sensitive, specific, simple and rapid analysis, etc.

Description

1. Technical field [0001] The invention relates to a chromatographic analysis method, and specifically claims to be a high-performance liquid chromatographic method for determining the content of the active ingredient reserpine in plants of the genus Rauwolfia. 2. Background technology [0002] Reserpine is extracted from the roots of Rauvolfia plants in the family Apocynaceae. It is a natural medicine with hypotensive effect. The main preparations include "Reserpine Tablets", "Reserpine Injection", " Compound Jiangya Tablets", etc., the structural formula of reserpine is as follows: [0003] rserpine reserpine [0004] Due to the complex structure and high cost of chemical synthesis, reserpine is currently extracted from the plants of the genus Rauwolfia, and the main resources are R.yunnanensis and R.vomitoria. As resources are depleted due to years of digging, artificial cultivation is on the rise. There are few studies on the content analysis of reserpine in Rauwolf...

Claims

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Application Information

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IPC IPC(8): G01N30/02B01J20/286G01N30/26
Inventor 龚云麒张慧颖饶高雄蔡传涛
Owner 饶高雄
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